Team:Harvard/parts

From 2010.igem.org

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<tr><td>GF3</td><td>BBa_K382031</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td><td></td> </tr>
<tr><td>GF3</td><td>BBa_K382031</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td><td></td> </tr>
<tr><td>GF4</td><td>BBa_K38202</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr>
<tr><td>GF4</td><td>BBa_K38202</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr>
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<tr><td>GF5</td><td>BBa_K382033</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>RXRHm</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td><td>-</td></tr>
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<tr><td>GF5</td><td>RXRHm</td><td>BBa_K382033</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td><td>-</td></tr>
<tr><td>GF5><td> Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.</td><td>VP16</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f6">link</a></td></tr>
<tr><td>GF5><td> Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.</td><td>VP16</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f6">link</a></td></tr>
<tr><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr>
<tr><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr>

Revision as of 18:31, 25 October 2010



parts

NameBiobrick NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1BBa_K382000pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpat pORE O1
V2BBa_K382001pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptII pORE O2
V3BBa_K382002pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpat pORE E3
V4BBa_K382003pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptII pORE E4
V5BBa_K382004pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptII pORE R1
V6BBa_K382005pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptII pORE R3
V7BBa_K382006
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpat V7
V8BBa_K382007
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptII V8
V9BBa_K382008
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat V9
V10BBa_K382009
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII V10
V11BBa_K382010
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII V11
V12BBa_K382011
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII V12
A1BBa_K382012amiRNA GFPGFP knockdown constructchloramphenicol- amiRNA GFP
A2BBa_K382013amiRNA LTP LTP knockdown constructchloramphenicol- amiRNA LTP
A3BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol- ihpRNA Bet1
A4BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A5BBa_K382016ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F1BBa_K382017pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-
F2BBa_K382018NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-
F3BBa_K382019NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-
F4BBa_K382020BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-
F5BBa_K382021MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-
F6BBa_K382022Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-
F7BBa_K382023Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-
F8BBa_K382024Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-
F9BBa_K382025Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-
C1BBa_K382026amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2)knockdownchloramphenicol-amiRNA LUT2
C2BBa_K382027amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-amiRNA BETA-OHASE 1
C3BBa_K382028amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-amiRNA LYC
GF1BBa_K382029Barnase Fatal gene for the genetic fence chloramphenicollink
GF2BBa_K382030BarstarProtein inhibitor of Barnasechloramphenicollink
GF3BBa_K382031LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-link
GF4BBa_K38202RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozidechloramphenicol-link
GF5RXRHmBBa_K382033Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicollink-
GF5> Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.VP16chloramphenicollink
Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicollink
Gal4 DNA binding domain Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. chloramphenicollink
GF4BBa_K382032RXRLc-VP16 Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozidechloramphenicol-
GF5BBa_K382033EcR-Gal4 Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide chloramphenicol-
Barnase Fatal gene for the genetic fence chloramphenicollink
BarstarProtein inhibitor of Barnasechloramphenicollink
LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicollink
RXRLc Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozidechloramphenicollink
RXRHmHuman Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.chloramphenicollink
VP16 Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.chloramphenicollink
Ecdysone Receptor Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide chloramphenicollink
Gal4 DNA binding domain Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. chloramphenicollink


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