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Team:Mexico-UNAM-CINVESTAV/Notebook/Week Five
From 2010.igem.org
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==''Tuesday''== | ==''Tuesday''== | ||
| - | '''With a overnight inoculum we going to do a miniprep''' | + | '''With a overnight inoculum we going to do a miniprep.''' |
| - | '''The miniprep was done with a Quiagen Kit''' | + | '''The miniprep was done with a Quiagen Kit.''' |
| - | '''Then we going to do a duble digest with EcoRI and PstI to check out our ligations''' | + | '''Then we going to do a duble digest with EcoRI and PstI to check out our ligations.''' |
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|10μl | |10μl | ||
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| + | ==''Wensday''== | ||
Revision as of 16:20, 25 October 2010
Contents |
Week #5
04th October - 08 October 2010
Monday
- Let's picked up colonys our transformant cells and prepare all to do miniprep.
For this we used 30ml de LB liquid agar medium per falcon tube at 80μ/ml
Cloramphenicol concentration we left overnight culture to 37º
Tuesday
With a overnight inoculum we going to do a miniprep.
The miniprep was done with a Quiagen Kit.
Then we going to do a duble digest with EcoRI and PstI to check out our ligations.
| DNA | 5μl |
| Buffer NB2 | 1μl |
| EcoRI | 0.5μl |
| PstI | 0.5μl |
| H2O | 2.7μl |
| BSA | 0.3μl |
| Total | 10μl |
