Team:ETHZ Basel/Achievements/BioBrick Toolbox

From 2010.igem.org

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(→Chemotaxis BrixBox)
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= BioBrick Toolbox =
= BioBrick Toolbox =
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To construct the aimed fusion proteins, we first cloned our BioBricks into the storage vector pSEVA132 (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721).
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To construct the aimed fusion proteins, we first cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721).
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C-parts enable C-Terminal fusion and N-parts N-Terminal fusion.
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A-parts N-Terminal fusion and C-parts C-terminal fusion. B-part is the linker that connects the two entities.
== Chemotaxis BrixBox ==
== Chemotaxis BrixBox ==
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! Name
! Name
! Insert
! Insert
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! Backbone
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! Orientation
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! Name
 +
! Insert
 +
! Orientation
! Description
! Description
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! Favourite part
 +
|-
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| <partinfo>BBa_K422003</partinfo>
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| CheB
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| A-part
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| <partinfo>BBa_K422004</partinfo>
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| CheB
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| C-part
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| Demthylase: Demethylates methyl accepting chemotaxis proteins MCPs
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|
|-
|-
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| <partinfo>BBa_B0015</partinfo>
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| <partinfo>BBa_K422005</partinfo>
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| row 1, cell 1
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| CheR
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| row 1, cell 2
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| A-part
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| row 1, cell 3
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| <partinfo>BBa_K422006</partinfo>
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| CheR
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| C-part
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| Methyltransferase: Methylates methyl accepting chemotaxis proteins MCPs
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|  
|-
|-
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| <partinfo>BBa_B0015</partinfo>
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| <partinfo>BBa_K422007</partinfo>
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| row 2, cell 1
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| CheY
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| row 2, cell 2
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| A-part
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| row 2, cell 3
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| <partinfo>BBa_K422008</partinfo>
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| CheY
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| C-part
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| Induces tumbling by interacting with the flagellar switch protein FliM
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|  
|}
|}

Revision as of 13:58, 25 October 2010

BioBrick Toolbox

To construct the aimed fusion proteins, we first cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721). A-parts N-Terminal fusion and C-parts C-terminal fusion. B-part is the linker that connects the two entities.

Chemotaxis BrixBox

Name Insert Orientation Name Insert Orientation Description Favourite part
<partinfo>BBa_K422003</partinfo> CheB A-part <partinfo>BBa_K422004</partinfo> CheB C-part Demthylase: Demethylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422005</partinfo> CheR A-part <partinfo>BBa_K422006</partinfo> CheR C-part Methyltransferase: Methylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422007</partinfo> CheY A-part <partinfo>BBa_K422008</partinfo> CheY C-part Induces tumbling by interacting with the flagellar switch protein FliM

Anchor BrixBox

Ligth sensor BrixBox

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