Team:LMU-Munich/Jump-or-die/Functional Principle
From 2010.igem.org
(Difference between revisions)
(→d) both constructs 2 and 3 enter the cell) |
(→d) both constructs 2 and 3 enter the cell) |
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PhiC31o will be read off because of CMV promoter. Now the integrase will combine attB and attP site and integrate construct 2 into the genome. Now the gene of interest is integrated between the tet-on promoter and bak in the cellular genome. This complex will be translated after tetracycline induction. The doubled stop codon and polyadenylation site directly after eGFP (the gene of interest) will efficiently prevent the translation of bak. Thus such cells will express the gene of interest and survive. | PhiC31o will be read off because of CMV promoter. Now the integrase will combine attB and attP site and integrate construct 2 into the genome. Now the gene of interest is integrated between the tet-on promoter and bak in the cellular genome. This complex will be translated after tetracycline induction. The doubled stop codon and polyadenylation site directly after eGFP (the gene of interest) will efficiently prevent the translation of bak. Thus such cells will express the gene of interest and survive. | ||
- | [[Image: Dnajump4.PNG | | + | [[Image: Dnajump4.PNG | 600px | Jump recombination]] |
- | [[Image: Dnajump5.PNG | | + | [[Image: Dnajump5.PNG | 600px | Jump recombination]] |
- | [[Image: Dnajump6.PNG | | + | [[Image: Dnajump6.PNG | 600px | Jump recombination]] |
- | [[Image: Dnajump7.PNG | | + | [[Image: Dnajump7.PNG | 600px | Jump recombination]] |
Revision as of 10:54, 25 October 2010