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Team:Michigan/Media Recipes
From 2010.igem.org
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==Media Recipes== | ==Media Recipes== | ||
| - | Kanamycin Antibiotic Stock Solution (50 mg/mL) | + | '''Kanamycin Antibiotic Stock Solution (50 mg/mL)''' |
*250 mg of kanamycin | *250 mg of kanamycin | ||
*5 mL of DI water | *5 mL of DI water | ||
''filter sterilize and make 1 mL aliquots to store in the -20C freezer'' | ''filter sterilize and make 1 mL aliquots to store in the -20C freezer'' | ||
| - | Tetracycline Antibiotic Stock Solution (50 mg/mL) | + | '''Tetracycline Antibiotic Stock Solution (50 mg/mL)''' |
*250 mg of tetracycline | *250 mg of tetracycline | ||
*2.5 mL of DI water | *2.5 mL of DI water | ||
*2.5 mL of ethanol | *2.5 mL of ethanol | ||
''filter sterilize and make 1 mL aliquots to store in the -20C freezer'' | ''filter sterilize and make 1 mL aliquots to store in the -20C freezer'' | ||
Revision as of 02:41, 30 June 2010
Media Recipes
Kanamycin Antibiotic Stock Solution (50 mg/mL)
- 250 mg of kanamycin
- 5 mL of DI water
filter sterilize and make 1 mL aliquots to store in the -20C freezer
Tetracycline Antibiotic Stock Solution (50 mg/mL)
- 250 mg of tetracycline
- 2.5 mL of DI water
- 2.5 mL of ethanol
filter sterilize and make 1 mL aliquots to store in the -20C freezer
