Team:Tsinghua/Notebook/26 August 2010

From 2010.igem.org

(Difference between revisions)
(Module I, DT and Fan's part:)
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== Module I, group 2(b) ==
== Module I, group 2(b) ==
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The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure.
The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure.
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== Module I, Group 2c ==
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Ligation product is transformed in to DH5aα 24h after ligation.

Revision as of 20:27, 26 October 2010

Module I, group 2(b)

Run a colony PCR with universal primers. Amplify the bacteria at the same time.

PCR system (SuperMix):

 H2O	        8μl
 primer1	1μl
 primer2	1μl
 template	Some
 SuperMix	10μl
 Total	        20μl

result

10-8-26.jpg

The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure.

Module I, Group 2c

Ligation product is transformed in to DH5aα 24h after ligation.