Team:Tsinghua/Notebook/26 August 2010
From 2010.igem.org
(Difference between revisions)
(→Module I, DT and Fan's part:) |
|||
Line 1: | Line 1: | ||
- | |||
== Module I, group 2(b) == | == Module I, group 2(b) == | ||
Line 16: | Line 15: | ||
The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure. | The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure. | ||
+ | == Module I, Group 2c == | ||
+ | |||
+ | Ligation product is transformed in to DH5aα 24h after ligation. |
Revision as of 20:27, 26 October 2010
Module I, group 2(b)
Run a colony PCR with universal primers. Amplify the bacteria at the same time.
PCR system (SuperMix):
H2O 8μl primer1 1μl primer2 1μl template Some SuperMix 10μl Total 20μl
result
The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure.
Module I, Group 2c
Ligation product is transformed in to DH5aα 24h after ligation.