Team:Mexico-UNAM-CINVESTAV/Notebook/Week Five

From 2010.igem.org

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==''Tuesday''==
==''Tuesday''==
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'''With a inoculum overnight culture we going to do a miniprep'''
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'''With a overnight inoculum  we going to do a miniprep'''
'''The miniprep was done with a Quiagen Kit'''
'''The miniprep was done with a Quiagen Kit'''
-
'''Then we going to do a duble digestion with EcoRI and PstI to check out our ligations'''
+
'''Then we going to do a duble digest with EcoRI and PstI to check out our ligations'''

Revision as of 16:10, 25 October 2010


Contents

Week #5

04th October - 08 October 2010

Monday

  • Let's picked up colonys our transformant cells and prepare all to do miniprep.

For this we used 30ml de LB liquid agar medium per falcon tube at 80μ/ml

Cloramphenicol concentration we left overnight culture to 37º


Labo 271.JPG


Tuesday

With a overnight inoculum we going to do a miniprep

The miniprep was done with a Quiagen Kit

Then we going to do a duble digest with EcoRI and PstI to check out our ligations


DNA 5μl
Buffer NB2 1μl
EcoRI 0.5μl
PstI 0.5μl
H2O 2.7μl
BSA 0.3μl
Total 10μl