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Team:Harvard/parts
From 2010.igem.org
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<tr><td>C3</td><td>BBa_K382028</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr> | <tr><td>C3</td><td>BBa_K382028</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/f/f8/BBa_K382028_C3.txt">amiRNA LYC</a></td></tr> | ||
| - | <tr><td>GF1</td><td>BBa_K382029</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td> | + | <tr><td>GF1</td><td>BBa_K382029</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f2">link</a></td><td></td></tr> |
| - | <tr><td>GF2</td><td>BBa_K382030</td><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td> | + | <tr><td>GF2</td><td>BBa_K382030</td><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f2">link</a></td><td></td></tr> |
| - | <tr><td>GF3</td><td>BBa_K382031</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td></td> </tr> | + | <tr><td>GF3</td><td>BBa_K382031</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td><td></td> </tr> |
| - | <tr><td>GF4</td><td>BBa_K382032</td><td>RXRLc-VP16</td><td> Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td></td></tr> | + | <tr><td>GF4</td><td>BBa_K38202</td><td>RXRLc<td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr> |
| + | <tr><td>GF5</td><td>BBa_K382033</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>RXRHm</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td><td>-</td></tr> | ||
| + | <tr><td>GF5><td> Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.</td><td>VP16</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f6">link</a></td></tr> | ||
| + | <tr><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr> | ||
| + | <tr><td>Gal4 DNA binding domain</td><td> Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f8">link</a></td></tr> | ||
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| + | <tr><td>GF4</td><td>BBa_K382032</td><td>RXRLc-VP16</td><td> Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td></td></tr> | ||
<tr><td>GF5</td><td>BBa_K382033</td><td>EcR-Gal4</td><td> Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td></td></tr> | <tr><td>GF5</td><td>BBa_K382033</td><td>EcR-Gal4</td><td> Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td></td></tr> | ||
| + | <tr><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f1">link</a></td></tr> | ||
| + | <tr><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f2">link</a></td></tr> | ||
| + | <tr><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f3">link</a></td></tr> | ||
| + | <tr><td>RXRLc</td><td> Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f4">link</a></td></tr> | ||
| + | <tr><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr> | ||
| + | <tr><td>VP16</td><td> Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter.</td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f6">link</a></td></tr> | ||
| + | <tr><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr> | ||
| + | <tr><td>Gal4 DNA binding domain</td><td> Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. </td><td>chloramphenicol</td><td><a href="https://2010.igem.org/Team:Harvard/f8">link</a></td></tr> | ||
</table> | </table> | ||
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Revision as of 18:30, 25 October 2010
parts
| Name | Biobrick Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence | |
| V1 | BBa_K382000 | pORE Open Series 1 (O1) | Agrobacterium vector open series | Kanamycin | pat | pORE O1 | |
| V2 | BBa_K382001 | pORE Open Series 2 (O2) | Agrobacterium vector open series | Kanamycin | nptII | pORE O2 | |
| V3 | BBa_K382002 | pORE Expression Series 3 (E3) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | pat | pORE E3 | |
| V4 | BBa_K382003 | pORE Expression Series 4 (E4) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | nptII | pORE E4 | |
| V5 | BBa_K382004 | pORE Reporter Series 1 (R1) | Agrobacterium vector with gusA reporter | Kanamycin | nptII | pORE R1 | |
| V6 | BBa_K382005 | pORE Reporter Series 3 (R3) | Agrobacterium vector with smgfp reporter | Kanamycin | nptII | pORE R3 | |
| V7 | BBa_K382006 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | pat | V7 | ||
| V8 | BBa_K382007 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | nptII | V8 | ||
| V9 | BBa_K382008 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | pat | V9 | ||
| V10 | BBa_K382009 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | nptII | V10 | ||
| V11 | BBa_K382010 | Agrobacterium vector with gusA reporter and biobrick cloning site | Kanamycin | nptII | V11 | ||
| V12 | BBa_K382011 | Agrobacterium vector with smgfp reporter and biobrick cloning site | Kanamycin | nptII | V12 | ||
| A1 | BBa_K382012 | amiRNA GFP | GFP knockdown construct | chloramphenicol | - | amiRNA GFP | |
| A2 | BBa_K382013 | amiRNA LTP | LTP knockdown construct | chloramphenicol | - | amiRNA LTP | |
| A3 | BBa_K382014 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | - | ihpRNA Bet1 | |
| A4 | BBa_K382015 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | - | ihpRNA LTP | |
| A5 | BBa_K382016 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | - | ihpRNA GER | |
| F1 | BBa_K382017 | pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | chloramphenicol | - | ||
| F2 | BBa_K382018 | NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | chloramphenicol | - | ||
| F3 | BBa_K382019 | NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on 5' end. | chloramphenicol | - | ||
| F4 | BBa_K382020 | Brazzein | Sweet-tasting protein. 54 amino acid sequence. | chloramphenicol | - | ||
| F5 | BBa_K382021 | Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | chloramphenicol | - | ||
| F6 | BBa_K382022 | Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | ||
| F7 | BBa_K382023 | Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | ||
| F8 | BBa_K382024 | Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | ||
| F9 | BBa_K382025 | Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | ||
| C1 | BBa_K382026 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown | chloramphenicol | - | amiRNA LUT2 | |
| C2 | BBa_K382027 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | chloramphenicol | - | amiRNA BETA-OHASE 1 | |
| C3 | BBa_K382028 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | chloramphenicol | - | amiRNA LYC | |
| GF1 | BBa_K382029 | Barnase | Fatal gene for the genetic fence | chloramphenicol | link | ||
| GF2 | BBa_K382030 | Barstar | Protein inhibitor of Barnase | chloramphenicol | link | ||
| GF3 | BBa_K382031 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | - | link | |
| GF4 | BBa_K38202 | RXRLc | Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide | chloramphenicol | - | link | |
| GF5 | BBa_K382033 | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | chloramphenicol | RXRHm | link | - | |
| GF5> | Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter. | VP16 | chloramphenicol | link | |||
| Ecdysone Receptor | Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide | chloramphenicol | link | ||||
| Gal4 DNA binding domain | Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. | chloramphenicol | link | ||||
| GF4 | BBa_K382032 | RXRLc-VP16 | Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide | chloramphenicol | - | ||
| GF5 | BBa_K382033 | EcR-Gal4 | Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide | chloramphenicol | - | ||
| Barnase | Fatal gene for the genetic fence | chloramphenicol | link | ||||
| Barstar | Protein inhibitor of Barnase | chloramphenicol | link | ||||
| LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | link | ||||
| RXRLc | Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide | chloramphenicol | link | ||||
| RXRHm | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | chloramphenicol | link | ||||
| VP16 | Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter. | chloramphenicol | link | ||||
| Ecdysone Receptor | Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide | chloramphenicol | link | ||||
| Gal4 DNA binding domain | Part of the Gal4-EcR fusion protein. Gal4 associates with the Gal4 upstream activating sequence, and when RXR-VP16 is bound, it localizes VP16 to the Gal4UAS35s promoter resulting in transcription downstream. | chloramphenicol | link |
