"
Team:Groningen/9 August 2010
From 2010.igem.org
| Line 12: | Line 12: | ||
'''Modellers:''' Look up and read more articles on the ComXPA quorum sensing system. These pathways can get complicated so after reading a few articles we decided to be old fashioned and draw everything on the blackboard. ''Joël, Laura, Djoke'' | '''Modellers:''' Look up and read more articles on the ComXPA quorum sensing system. These pathways can get complicated so after reading a few articles we decided to be old fashioned and draw everything on the blackboard. ''Joël, Laura, Djoke'' | ||
| + | |||
| + | '''Geeske''' | ||
| + | |||
| + | Transformed ''E. coli'' TOP10 with: | ||
| + | |||
| + | -pSB1C3_J04450 (RFP) (plate 1 well 3A 2010 distribution) | ||
| + | |||
| + | -pSB1K3_J04450 (RFP) (plate 1 well 5A 2010 distribution) | ||
| + | |||
| + | These were used to produce pSB1C3_H and pSB1K3_EH via E.coli. Selfclosers would turn red and could easily be ignored, making isolating the backbone from gel unnecessary. Plasmids were checked by restriction. | ||
Revision as of 19:15, 26 October 2010
Hydrophobofilm
pushing coatings into a greener future
Week 32
Cell wall isolation streptomyces- Peter
Degredation experiment-Peter
Modellers: Look up and read more articles on the ComXPA quorum sensing system. These pathways can get complicated so after reading a few articles we decided to be old fashioned and draw everything on the blackboard. Joël, Laura, Djoke
Geeske
Transformed E. coli TOP10 with:
-pSB1C3_J04450 (RFP) (plate 1 well 3A 2010 distribution)
-pSB1K3_J04450 (RFP) (plate 1 well 5A 2010 distribution)
These were used to produce pSB1C3_H and pSB1K3_EH via E.coli. Selfclosers would turn red and could easily be ignored, making isolating the backbone from gel unnecessary. Plasmids were checked by restriction.
