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Revision as of 20:13, 28 June 2010 (view source) Gcxmatt (Talk | contribs) (→Live/Dead E.Coli Assay) ← Older edit		Revision as of 21:04, 16 October 2010 (view source) Cyu286 (Talk | contribs) Newer edit →
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-	===T4 Holin===	+	We used the Apoptosis Cassette by Brown '08 iGEM team (K124017).
-	===Endolycin===	+	The bacteriophage cassette includes S105 protein (Holin), R protein (Endolysin), and Rz protein, all of which act in combination to lyse the bacteria.
-	===Rz Protein===	+	Holin is a small membrane protein that produces holes in the membrane.
-	===Live/Dead E.Coli Assay===	+	R is an endolysin that digests and cleaves the cell wall.
-	Fluoresceinediacetate = stains living cells	+	Rz protein, a periplasmic protein, through reasons that are not yet totally clear, carries out the final step of host lysis.
-		+
-	Ethidium Bromide = stains dead cells	+

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Revision as of 21:04, 16 October 2010

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We used the Apoptosis Cassette by Brown '08 iGEM team (K124017).

The bacteriophage cassette includes S105 protein (Holin), R protein (Endolysin), and Rz protein, all of which act in combination to lyse the bacteria.

Holin is a small membrane protein that produces holes in the membrane.

R is an endolysin that digests and cleaves the cell wall.

Rz protein, a periplasmic protein, through reasons that are not yet totally clear, carries out the final step of host lysis.