Team:Yale/Our Project/Protocols/bacteria mixture for H2S Assay

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<h4> Bacteria Mixture for H2S Assay </h4>
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our project
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<li><a href="https://2010.igem.org/Team:Yale/Our Project">introduction</a></li>
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protocols
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<h4> Bacteria Mixture for H<sub>2</sub>S Assay </h4>
In order to facilitate the manipulation of E.coli, a solution was made using:<br/>
In order to facilitate the manipulation of E.coli, a solution was made using:<br/>
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<li> 10 microliters of IPTG </li>
<li> 10 microliters of IPTG </li>
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<li> 200 microliters of S.O.C. (a medium that promotes growth)</li>
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<li> 200 microliters of SOC (link)</li>
<li> E.coli cultures </li>
<li> E.coli cultures </li>
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<br /> This mixture was then vortexed to create a thick liquid of bacteria. <br />
<br /> This mixture was then vortexed to create a thick liquid of bacteria. <br />
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Latest revision as of 21:07, 24 October 2010

iGEM Yale

protocols

Bacteria Mixture for H2S Assay

In order to facilitate the manipulation of E.coli, a solution was made using:
  • 10 microliters of IPTG
  • 200 microliters of SOC (link)
  • E.coli cultures

This mixture was then vortexed to create a thick liquid of bacteria.

Retrieved from "http://2010.igem.org/Team:Yale/Our_Project/Protocols/bacteria_mixture_for_H2S_Assay"