Team:Yale/Our Project/Protocols
From 2010.igem.org
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<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/LB_agar">LB agar</a> <br/> | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/LB_agar">LB agar</a> <br/> | ||
<a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar">Triple Sugar Iron (TSI) agar</a> | <a id="link" href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar">Triple Sugar Iron (TSI) agar</a> | ||
+ | |||
+ | <br /> The composition of the plates were:<br /> | ||
+ | |||
+ | <br /><li>500 mL water</a></li> | ||
+ | <li>1.5 g Meat Extract </a></li> | ||
+ | <li>1.5 g Yeast Extract</a></li> | ||
+ | <li>7.5 g Pancreatic Digest of Casein</a></li> | ||
+ | <li> 2.5 g Protease Peptone</a></li> | ||
+ | <li>0.5 g Dextrose</a></li> | ||
+ | <li>0.5 g Lactose</a></li> | ||
+ | <li>0.5 g Sucrose</a></li> | ||
+ | <li>0.26 g Ferrous Ammonium Sulfate (hexahydrate)</a></li> | ||
+ | <li>2.5 g NaCl</a></li> | ||
+ | <li>0.15 g Sodium Thiosulfate</a></li> | ||
+ | <li>6 g Agar</a></li> | ||
+ | <li>12 mg Phenol Red</a></li> | ||
+ | <li>+ Ampicillin</a></li> | ||
<h4> Copper absorbance protocols </h4> | <h4> Copper absorbance protocols </h4> |
Revision as of 19:19, 24 October 2010
our project
protocols
Bacterial protocols
transformationDNA protocols
miniprepgel extraction
PCR purification
ethanol purification
Recipes
MOPS minimal mediaSOC medium
LB agar
Triple Sugar Iron (TSI) agar
The composition of the plates were:
Copper absorbance protocols
standard absorbance curve measurementcopper disappearance as a function of bacterial growth curve