Team:Harvard/parts

From 2010.igem.org

(Difference between revisions)
(fixed error in numbering)
Line 58: Line 58:
<tr><td>F9</td><td>BBa_K382025</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td></td></tr>
<tr><td>F9</td><td>BBa_K382025</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td></td></tr>
<tr><td>C1</td><td>BBa_K382026</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr>
<tr><td>C1</td><td>BBa_K382026</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr>
-
<tr><td>C2</td><td>BBa_K382026</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr>
+
<tr><td>C2</td><td>BBa_K382027</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr>
-
<tr><td>C3</td><td>BBa_K382027</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr>
+
<tr><td>C3</td><td>BBa_K382028</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr>
-
<tr><td>GF1</td><td>BBa_K382028</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td>-</td><td></td></tr>
+
<tr><td>GF1</td><td>BBa_K382029</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td>-</td><td></td></tr>
-
<tr><td>GF2</td><td>BBa_K382029</td><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td>-</td><td></td></tr>
+
<tr><td>GF2</td><td>BBa_K382030</td><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td>-</td><td></td></tr>
-
<tr><td>GF3</td><td>BBa_K382030</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td></td> </tr>
+
<tr><td>GF3</td><td>BBa_K382031</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td>-</td><td></td> </tr>
-
<tr><td>GF4</td><td>BBa_K382031</td><td>RXRLc-VP16</td><td> Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td></td></tr>
+
<tr><td>GF4</td><td>BBa_K382032</td><td>RXRLc-VP16</td><td> Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td></td></tr>
-
<tr><td>GF5</td><td>BBa_K382032</td><td>EcR-Gal4</td><td> Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td></td></tr>
+
<tr><td>GF5</td><td>BBa_K382033</td><td>EcR-Gal4</td><td> Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td></td></tr>
</table>
</table>
<br /><br />
<br /><br />

Revision as of 19:11, 24 October 2010

parts and primers

NameBiobrick NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1BBa_K382000pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpat pORE O1
V2BBa_K382001pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptII pORE O2
V3BBa_K382002pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpat pORE E3
V4BBa_K382003pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptII pORE E4
V5BBa_K382004pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptII pORE R1
V6BBa_K382005pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptII pORE R3
V7BBa_K382006
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpat V7
V8BBa_K382007
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptII V8
V9BBa_K382008
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat V9
V10BBa_K382009
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII V10
V11BBa_K382010
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII V11
V12BBa_K382011
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII V12
A1BBa_K382012amiRNA GFPGFP knockdown constructchloramphenicol- amirnaGFP
A2BBa_K382013amiRNA LTP LTP knockdown constructchloramphenicol- amiRNA LTP
A3BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol- ihpRNA Bet1
A4BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A5BBa_K382016ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F1BBa_K382017pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants. chloramphenicol-
F2BBa_K382018NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.chloramphenicol-
F3BBa_K382019NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on 5' end.chloramphenicol-
F4BBa_K382020BrazzeinSweet-tasting protein. 54 amino acid sequence. chloramphenicol-
F5BBa_K382021MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequencechloramphenicol-
F6BBa_K382022Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-
F7BBa_K382023Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expressionchloramphenicol-
F8BBa_K382024Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.chloramphenicol-
F9BBa_K382025Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.chloramphenicol-
C1BBa_K382026amiRNA LUT2amiRNA construct for lycopene epsilon cyclase (LUT2)knockdownchloramphenicol-
C2BBa_K382027amiRNA BETA-OHASE 1amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdownchloramphenicol-
C3BBa_K382028amiRNA LYCamiRNA construct for lycopene beta cyclase (LYC) knockdownchloramphenicol-
GF1BBa_K382029Barnase Fatal gene for the genetic fence chloramphenicol-
GF2BBa_K382030BarstarProtein inhibitor of Barnasechloramphenicol-
GF3BBa_K382031LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol-
GF4BBa_K382032RXRLc-VP16 Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozidechloramphenicol-
GF5BBa_K382033EcR-Gal4 Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide chloramphenicol-


Retrieved from "http://2010.igem.org/Team:Harvard/parts"