Team:Michigan/Project
From 2010.igem.org
(Difference between revisions)
(→Hy-Bi: Virus Protein Surface Display) |
(→Hy-Bi: Virus Protein Surface Display) |
||
Line 49: | Line 49: | ||
We devised two ways to cause flocculation, one being using a pili expression and the other using a virus surface protein, and the subject of our group is the virus surface protein. During its viral attack on cells, virus needs a protein that enables it to attach to the surface of the host cells. One of the virus surface proteins, vp 130, is used by chlorovirus to attach itself onto the surface of algae. Onimatsu, et al. recombined the vp 130 gene from Chlorovirus CVK2 with a plasmid, producing a wealth of vp 130. The binding of these proteins on their host cells, chlorella, was detected using fluorescent vp 130 specific antibodies (fig.1)[7]. | We devised two ways to cause flocculation, one being using a pili expression and the other using a virus surface protein, and the subject of our group is the virus surface protein. During its viral attack on cells, virus needs a protein that enables it to attach to the surface of the host cells. One of the virus surface proteins, vp 130, is used by chlorovirus to attach itself onto the surface of algae. Onimatsu, et al. recombined the vp 130 gene from Chlorovirus CVK2 with a plasmid, producing a wealth of vp 130. The binding of these proteins on their host cells, chlorella, was detected using fluorescent vp 130 specific antibodies (fig.1)[7]. | ||
- | |||
[[Image:vp130.jpg|450px|right|thumb|fig.2 a model of vp 130-expressed bacteria binding algae cells]] | [[Image:vp130.jpg|450px|right|thumb|fig.2 a model of vp 130-expressed bacteria binding algae cells]] |
Revision as of 16:02, 24 October 2010