Team:Michigan/Project
From 2010.igem.org
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[[Image:vp130.jpg|450px|right|thumb|fig.2 a model of vp 130-expressed bacteria binding algae cells]] | [[Image:vp130.jpg|450px|right|thumb|fig.2 a model of vp 130-expressed bacteria binding algae cells]] | ||
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So, if we transfer the gene for this surface binding protein from the virus to E. Coli., each bacteria cell will bind to multiple algae cells, which bind to more bacteria, causing aggregation and flocculation. As opposed to using pili as the means to cause flocculation, the advantage of vp130 is its specific binding and aggregation of algae, automatically yielding a high algae mass in the flocculate (fig.2). However, there are also disadvantages. For example, vp 130 is not a E. Coli surface protein, so it must be cloned as a fusion to a known surface protein. We also suspected OmpA and ice nucleation protein (INP) as candidate surface proteins. | So, if we transfer the gene for this surface binding protein from the virus to E. Coli., each bacteria cell will bind to multiple algae cells, which bind to more bacteria, causing aggregation and flocculation. As opposed to using pili as the means to cause flocculation, the advantage of vp130 is its specific binding and aggregation of algae, automatically yielding a high algae mass in the flocculate (fig.2). However, there are also disadvantages. For example, vp 130 is not a E. Coli surface protein, so it must be cloned as a fusion to a known surface protein. We also suspected OmpA and ice nucleation protein (INP) as candidate surface proteins. | ||
Revision as of 16:01, 24 October 2010