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Team:Cambridge/BioBricks
From 2010.igem.org
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{{:Team:Cambridge/Templates/Tablerow|3d=905|title=luxCDEG pLambda AB|description=This part constitutively expresses [http://partsregistry.org/wiki/index.php?title=Part:BBa_K216008 the Xenorhabdus luminescens luxAB luciferase] already in the registry. It also features a translational unit for the substrate generating luxCDEG from Vibrio fischeri. This means that only when supplied with PoPs will it produce light output. }} | {{:Team:Cambridge/Templates/Tablerow|3d=905|title=luxCDEG pLambda AB|description=This part constitutively expresses [http://partsregistry.org/wiki/index.php?title=Part:BBa_K216008 the Xenorhabdus luminescens luxAB luciferase] already in the registry. It also features a translational unit for the substrate generating luxCDEG from Vibrio fischeri. This means that only when supplied with PoPs will it produce light output. }} | ||
{{:Team:Cambridge/Templates/Tablerow|3d=906|title=pBAD luxCDEG pLambda AB|description=This is the same as the part above but was built for testing induction by adding the pBAD promoter, making light inducible by addition of L-arabinose. }} | {{:Team:Cambridge/Templates/Tablerow|3d=906|title=pBAD luxCDEG pLambda AB|description=This is the same as the part above but was built for testing induction by adding the pBAD promoter, making light inducible by addition of L-arabinose. }} | ||
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| + | </table> | ||
| + | </html> | ||
| + | =Firefly luciferases= | ||
| + | <html> | ||
| + | <table style="background:transparent" class="partlist"> | ||
| + | </html> | ||
| + | {{:Team:Cambridge/Templates/Tablerow|3d=101|title=Luciferase from Photinus pyralis with increased substrate affinity|description=This is a translational unit for a mutated and codon-optimised form of the luciferase from the North American firefly, Photinus pyralis. It is described in [LINK LINK] as having a xxxxx affinity compared to wildtype.}} | ||
| + | {{:Team:Cambridge/Templates/Tablerow|3d=108|title=P.pyralis luciferase under pBAD|description=This is the same as the part above but placed under the pBAD promoter induced by L-arabinose for characterisation.}} | ||
| + | {{:Team:Cambridge/Templates/Tablerow|3d=211|title=L. cruciata luciferase (red mutant)|description=This is a translational unit for a mutant (Ser286Asn) and codon-optimised luciferase from the Japanese firefly, Luciola cruciata. It is deeply red-shifted as compared to wild type. }} | ||
| + | {{:Team:Cambridge/Templates/Tablerow|3d=218|title=L. cruciata luciferase under pBAD|description=This is the same as the part above but placed under the pBAD promoter induced by L-arabinose for characterisation.}} | ||
<html> | <html> | ||
</table> | </table> | ||
Revision as of 15:15, 24 October 2010
BioBricks
Characterisation data is stored in the Parts Registry. Click on any BioBrick to view this data.
<groupparts>iGEM010 Cambridge</groupparts>
Featured parts
 BBa_K325219 |
Red luciferase and LRE operon from L. cruciata under pBADThis part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, Luciola cruciata, when induced by L-arabinose. The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type. The sequence of nucleotides is codon optimised for expression in E. coli. |
|
BBa_K325909 |
V. fischeri luxCDABE under pBADThis part generates luxCDABE proteins from the bioluminescent bacterium, Vibrio fischeri, when induced by L-arabinose. The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence. The part creates a blue light. |
Parts based on bacterial systems
|
BBa_K325902 |
V. fischeri luxCDThis is a translational unit designed to produce V. fischeri luxC and luxD genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. Both proteins are part of the fatty aldehyde synthesis pathway. luxC is a reductase and luxD is a acytransferase. LuxE must also be supplied to complete the pathway for fatty aldehyde synthesis. Such fatty aldehydes are the substrate for the luxAB bacterial luciferase. |
|
BBa_K325903 |
V. fischeri luxEGThis is a translational unit designed to produce V. fischeri luxE and luxG genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. luxE functions as the synthetase creating fatty aldehydes for bioluminescence. LuxG is a flavin reductase. |
|
BBa_K325905 |
luxCDEG pLambda ABThis part constitutively expresses [http://partsregistry.org/wiki/index.php?title=Part:BBa_K216008 the Xenorhabdus luminescens luxAB luciferase] already in the registry. It also features a translational unit for the substrate generating luxCDEG from Vibrio fischeri. This means that only when supplied with PoPs will it produce light output. |
|
BBa_K325906 |
pBAD luxCDEG pLambda ABThis is the same as the part above but was built for testing induction by adding the pBAD promoter, making light inducible by addition of L-arabinose. |
Firefly luciferases
|
BBa_K325101 |
Luciferase from Photinus pyralis with increased substrate affinityThis is a translational unit for a mutated and codon-optimised form of the luciferase from the North American firefly, Photinus pyralis. It is described in [LINK LINK] as having a xxxxx affinity compared to wildtype. |
|
BBa_K325108 |
P.pyralis luciferase under pBADThis is the same as the part above but placed under the pBAD promoter induced by L-arabinose for characterisation. |
|
BBa_K325211 |
L. cruciata luciferase (red mutant)This is a translational unit for a mutant (Ser286Asn) and codon-optimised luciferase from the Japanese firefly, Luciola cruciata. It is deeply red-shifted as compared to wild type. |
|
BBa_K325218 |
L. cruciata luciferase under pBADThis is the same as the part above but placed under the pBAD promoter induced by L-arabinose for characterisation. |
