Team:Cambridge/BioBricks

From 2010.igem.org

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<h2 class="noline"><a href="http://partsregistry.org/Part:BBa_K325219">Red luciferase and LRE operon from L. cruciata under pBAD</a></h2>
<h2 class="noline"><a href="http://partsregistry.org/Part:BBa_K325219">Red luciferase and LRE operon from L. cruciata under pBAD</a></h2>
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This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, ''Luciola cruciata'', when induced by L-arabinose.  The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type.  The sequence of nucleotides is codon optimised for expression in E. coli.
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This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, <i>Luciola cruciata</i>, when induced by L-arabinose.  The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type.  The sequence of nucleotides is codon optimised for expression in E. coli.
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<a href="http://partsregistry.org/Part:BBa_K325219">
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<img src="https://static.igem.org/mediawiki/2010/a/a6/Cambridge-Lego.png"><br />BBa_K325219</a>
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<h2 class="noline"><a href="http://partsregistry.org/Part:BBa_K325219">V. fischeri luxCDABE under pBAD</a></h2>
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This part generates luxCDABE proteins from the bioluminescent bacterium, <i>Vibrio fischeri</i>, when induced by L-arabinose.  The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence.  The part creates a blue light.
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Revision as of 14:34, 24 October 2010