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Team:Cambridge/BioBricks
From 2010.igem.org
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<h2 class="noline"><a href="http://partsregistry.org/Part:BBa_K325219">Red luciferase and LRE operon from L. cruciata under pBAD</a></h2> | <h2 class="noline"><a href="http://partsregistry.org/Part:BBa_K325219">Red luciferase and LRE operon from L. cruciata under pBAD</a></h2> | ||
| - | This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, Luciola cruciata, when induced by arabinose. The luciferase produced has a point mutation Ser286Asn. The sequence of nucleotides is codon optimised for expression in E. coli. | + | This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, ''Luciola cruciata'', when induced by L-arabinose. The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type. The sequence of nucleotides is codon optimised for expression in E. coli. |
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Revision as of 14:24, 24 October 2010
BioBricks
Favourite parts
 BBa_K325219 |
Red luciferase and LRE operon from L. cruciata under pBADThis part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, ''Luciola cruciata'', when induced by L-arabinose. The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type. The sequence of nucleotides is codon optimised for expression in E. coli. |
