Team:Northwestern/Project

From 2010.igem.org

(Difference between revisions)
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http://openwetware.org/wiki/IPTG
http://openwetware.org/wiki/IPTG
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=== [Image:nuchitin.jpg]] Chitin Synthase ===
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=== [[Image:nuchitin.jpg]] Chitin Synthase ===
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311

Revision as of 04:01, 28 June 2010

Tech Institute

Home Team Project Parts Notebook Safety Calendar Protocol


Contents

Overall project

Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)

in chitinase-KO strain e coli

Project Details

Nulac.jpg Lac Repressor Signaling

http://openwetware.org/wiki/IPTG

Nuchitin.jpg Chitin Synthase

chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311

[Image:nuapop.jpg]] Holin

registry: http://partsregistry.org/Cell_death

Volatile Cell Signalling

phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA

article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411

Non-Volatile Cell Signalling

registry: http://partsregistry.org/Cell-cell_signalling

quorum sensing: LuxR, LuxS, LuxpR

AI-2 Purification

http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html

Chitinase Knockout (may not be necessary)

summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts

chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837

chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300

Parts

RBS
BBa_B0029 TCTAGAGTTCACACAGGAAACCTACTAGATG - 0.764
BBa_B0030 TCTAGAGATTAAAGAGGAGAAATACTAGATG 0.6 -
BBa_B0031 TCTAGAGTCACACAGGAAACCTACTAGATG 0.07 -
BBa_B0032 TCTAGAGTCACACAGGAAAGTACTAGATG 0.3 0.376
BBa_B0033 TCTAGAGTCACACAGGACTACTAGATG 0.01 0.002
BBa_B0034 TCTAGAGAAAGAGGAGAAATACTAGATG 1 1
BBa_B0035 TCTAGAGATTAAAGAGGAGAATACTAGATG - 1.124
BBa_B0064 TCTAGAGAAAGAGGGGAAATACTAGATG 0.35 -
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