Team:UNIPV-Pavia/Team/WwdPavia
From 2010.igem.org
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'''Manuel Zocco''' worked on: the construction of phasin fusion proteins | '''Manuel Zocco''' worked on: the construction of phasin fusion proteins | ||
- | (with Sara and '''Federica''') and performed the experiments on PHB/phasin | + | (with '''Sara''' and '''Federica''') and performed the experiments on PHB/phasin |
binding (with '''Federica'''). He also helped during validation experiments | binding (with '''Federica'''). He also helped during validation experiments | ||
by PCR on the integrative vector for E. coli and he had the idea (with | by PCR on the integrative vector for E. coli and he had the idea (with | ||
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- | + | ||
+ | ==Who did what...== | ||
''Integrative vector for E. coli:'' | ''Integrative vector for E. coli:'' | ||
- | *Manuel L. and Manuel Z. had the idea. | + | *'''Manuel L.''' and '''Manuel Z.''' had the idea. |
- | *Nicolò, and Matteo (supervised by Lorenzo) designed the vector and | + | *'''Nicolò''', and '''Matteo''' (supervised by '''Lorenzo''') designed the vector and |
the experiments. | the experiments. | ||
- | *Nicolò and Federica (supervised by Lorenzo and Matteo) constructed | + | *'''Nicolò''' and '''Federica''' (supervised by '''Lorenzo''' and '''Matteo''') constructed |
the clones and performed all the integration experiments. | the clones and performed all the integration experiments. | ||
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''Integrative vector for yeast:'' | ''Integrative vector for yeast:'' | ||
- | *Federica had the idea, designed the vector, cloned the vector in E. | + | *'''Federica''' had the idea, designed the vector, cloned the vector in ''E. |
- | coli, designed and performed integration experiments in S. cerevisiae. | + | coli'', designed and performed integration experiments in ''S. cerevisiae''. |
- | *Susanna helped Federica during the genome analysis of S288C strain | + | *'''Susanna''' helped '''Federica''' during the genome analysis of S288C strain |
to design the two integration sequences. | to design the two integration sequences. | ||
- | *Susanna and Manuel L. supervised the validation experiments. | + | *'''Susanna''' and '''Manuel L.''' supervised the validation experiments. |
''Self-inducible devices:'' | ''Self-inducible devices:'' | ||
- | *Lorenzo, Susanna and Nicolò had the idea. | + | *'''Lorenzo''', '''Susanna''' and '''Nicolò''' had the idea. |
- | *Nicolò (supervised by Susanna) designed and constructed all the | + | *'''Nicolò''' (supervised by '''Susanna''') designed and constructed all the |
- | devices. Nicolò also designed (supervised by Matteo and Susanna) and | + | devices. '''Nicolò''' also designed (supervised by '''Matteo''' and '''Susanna''') and |
- | performed (supervised by Susanna) all the experiments. | + | performed (supervised by '''Susanna''') all the experiments. |
- | *Nicolò analyzed the data. | + | *'''Nicolò''' analyzed the data. |
''PHB/Phasin binding:'' | ''PHB/Phasin binding:'' | ||
- | *Sara and Alessandro had the idea. | + | *'''Sara''' and '''Alessandro''' had the idea. |
- | *Sara and Alessandro (supervised by Giacomo) designed the phasin | + | *'''Sara''' and '''Alessandro''' (supervised by '''Giacomo''') designed the phasin |
fusion proteins. | fusion proteins. | ||
- | *Giacomo and Manuel L. supervised the construction of the clones for | + | *'''Giacomo''' and '''Manuel L.''' supervised the construction of the clones for |
- | validation. The physical construction was performed by Sara, Federica | + | validation. The physical construction was performed by '''Sara''', '''Federica''' |
- | and Manuel Z. | + | and '''Manuel Z.'''. |
- | *Sara and Federica (supervised by Susanna and Manuel L.) designed and | + | *'''Sara''' and '''Federica''' (supervised by '''Susanna''' and '''Manuel L.''') designed and |
performed the experiments on PHB. | performed the experiments on PHB. | ||
- | *Federica and Manuel Z. (supervised by Susanna and Giacomo) performed | + | *'''Federica''' and '''Manuel Z.''' (supervised by '''Susanna''' and '''Giacomo''') performed |
the experiments on phasin fusion proteins. | the experiments on phasin fusion proteins. | ||
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''Intein:'' | ''Intein:'' | ||
- | *Alessandro had the idea. | + | *'''Alessandro''' had the idea. |
- | *Sara designed the intein sequence. | + | *'''Sara''' designed the intein sequence. |
- | *Sara and Alessandro (supervised by Giacomo) designed the intein | + | *'''Sara''' and '''Alessandro''' (supervised by '''Giacomo''') designed the intein |
fusion proteins. | fusion proteins. | ||
- | *Giacomo supervised the construction of the clones for validation. | + | *'''Giacomo''' supervised the construction of the clones for validation. |
The physical construction was performed by Sara and Federica. | The physical construction was performed by Sara and Federica. | ||
- | *Nicolò (supervised by Giacomo) performed the experiments on intein | + | *'''Nicolò''' (supervised by '''Giacomo''') performed the experiments on intein |
fusion proteins. | fusion proteins. | ||
Revision as of 09:03, 24 October 2010
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