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-	*'''We have a meeting with our Genomics frend’s we going to get a	+	*'''We have had a meeting with our Genomics frend’s we going to get a'''
-	'''colaboration on Modeling and probably at wet lab too.	+	'''colaboration on Modeling and probably at wet lab too.'''

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Revision as of 20:20, 24 October 2010

Contents 1 Week 3 18th September -22th 2010 1.1 Monday 1.1.1 Planned and prepared for tomorrow's electroporation 1.1.2 We were looking for a nanodrop to quantify DNA’s concentrations 1.1.3 and tried to make ligations Psb1C3 with each one of primers 1.1.4 (our this year's bricks). 1.2 Tuesday 1.2.1 Transformed Top 10 competent cells, and did 10 plates incubated overnight. 1.3 Wednsday 1.3.1 Results previous day transformations only two plates were completed unsucessful. 1.4 Thursday 1.4.1 The inoculum for miniprep we prepared 6 vector’s vials 1.4.2 without positive results. Maybe the solutions for alcaline 1.4.3 lisis are not well.

Week 3 18th September -22th 2010

Monday

Planned and prepared for tomorrow's electroporation

• Depart electroporator.
• Made up LB agar plates and cloramphenicol 100μl.
• Plan to transform Psb1C3 froma plate.

We were looking for a nanodrop to quantify DNA’s concentrations

and tried to make ligations Psb1C3 with each one of primers

(our this year's bricks).

Tuesday

Transformed Top 10 competent cells, and did 10 plates incubated overnight.

Wednsday

Results previous day transformations only two plates were completed unsucessful.

• Prepared solutions for miniprep via Alcalin lisis method

Thursday

The inoculum for miniprep we prepared 6 vector’s vials

without positive results. Maybe the solutions for alcaline

lisis are not well.

• We have discused over how to quantify our cold sistem response

we gonig to use a promoter sistem, the funcional casset using J13002 as follow

• Promoter + our functional secuence + GFP

• Promoter + our functional secuence + AFP (Anty freeze protein)

• We have had a meeting with our Genomics frend’s we going to get a

colaboration on Modeling and probably at wet lab too.