"

From 2010.igem.org

Revision as of 23:49, 23 October 2010 (view source) AndreasConstantinou (Talk | contribs) (→Cell-penetrating peptides) ← Older edit		Revision as of 23:50, 23 October 2010 (view source) AndreasConstantinou (Talk | contribs) (→Cell-penetrating peptides) Newer edit →
Line 1:		Line 1:
	{{Stockholm/Results}}		{{Stockholm/Results}}
-
-	==Cell-penetrating peptides==
	===Low Molecular Weight Protamine (LMWP)===		===Low Molecular Weight Protamine (LMWP)===

---

Revision as of 23:50, 23 October 2010

Contents 1 Low Molecular Weight Protamine (LMWP) 1.1 Standard part 1.2 N-part 2 Transportan 10 (Tra10) 2.1 Standard part 2.2 N-part 3 Trans-Activating Transcriptional Activator (TAT) 3.1 Standard part 3.2 N-part 4 Proteins 4.1 Immunoglobulin G protease (IdeS) 4.2 Protein A Z-domain (ProtA-Z) 4.3 Superoxide dismutase (SOD) 4.3.1 Yeast Copper Chaperone (yCCS) 4.4 Basic Fibroblast Growth Factor (bFGF)

Low Molecular Weight Protamine (LMWP)

Standard part

Part name:

Registry name:

Sequencing results:

Functionality: Not verified

N-part

Part name:

Registry name:

Sequencing results:

Functionality: Not verified

Transportan 10 (Tra10)

Standard part

Part name:

Registry name:

Sequencing results:

Functionality: Not verified

N-part

Part name:

Registry name:

Sequencing results:

Functionality: Not verified

Trans-Activating Transcriptional Activator (TAT)

TAT is a cell-penetrating peptide that may be used in fusions with full-length proteins to create transduction proteins with the ability to permeate the lipid bilayer of various cell types.

Purified full-length TAT fusion proteins expressed in Escherichia coli have been shown to successfully translocate into several human cell types, including all cells found in whole blood, as well as bone marrow stem cells and osteoblasts, while still retaining the fused protein's activity ([http://www.ncbi.nlm.nih.gov/pubmed/9846587 Nagahara et al. 1998]). The mechanism for transduction over the bilipid membrane is still a matter of debate, but has been suggested to occur through macropinocytosis, a specialized form of endocytosis ([http://www.ncbi.nlm.nih.gov/pubmed/17913584 Gump and Dowdy, 2007]).

Standard part

Part name: TAT cell-penetrating peptide

Registry name: [http://partsregistry.org/Part:BBa_K380000 BBa_K380000]

Sequencing results: Verified (fasta)

Functionality: Not verified

N-part

Part name: N-part TAT cell-penetrating peptide

Registry name: [http://partsregistry.org/Part:BBa_K380000 BBa_K380001]

Sequencing results: Verified (fasta/Blastn)

Functionality: Not verified

Proteins

Immunoglobulin G protease (IdeS)

Protein A Z-domain (ProtA-Z)

Superoxide dismutase (SOD)

Yeast Copper Chaperone (yCCS)

Basic Fibroblast Growth Factor (bFGF)