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Team:Wisconsin-Madison/parts
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==New Standards== | ==New Standards== | ||
Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following: | Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following: | ||
| - | * Genbank including features and plasmid (.gbk | + | * Genbank including features and plasmid (.gbk) |
* Fwd and Reverse sequencing files (.ab1) | * Fwd and Reverse sequencing files (.ab1) | ||
* Gels from cloning and confirmation (.pdf) | * Gels from cloning and confirmation (.pdf) | ||
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|Produces RFP | |Produces RFP | ||
|RamA/Bile-Salt Complex | |RamA/Bile-Salt Complex | ||
| - | |[[ | + | |[[Media:Zip_file_acrRA.zip]] |
|- | |- | ||
|<partinfo>BBa_k318517</partinfo> | |<partinfo>BBa_k318517</partinfo> | ||
|Produces RamA gene | |Produces RamA gene | ||
|Bile Salt | |Bile Salt | ||
| - | |[[ | + | |[[Media:Zip_file_ramA.zip]] |
|} | |} | ||
<br> | <br> | ||
| Line 177: | Line 177: | ||
|Key Cassette - produces Cre and Hin recombinases | |Key Cassette - produces Cre and Hin recombinases | ||
|Arabinose induces Cre expression, IPTG induces Hin expression | |Arabinose induces Cre expression, IPTG induces Hin expression | ||
| - | |[[ | + | |[[Media:BBa_K318000_pSB1C3.zip]] |
|- | |- | ||
|<partinfo>BBa_K318030</partinfo> | |<partinfo>BBa_K318030</partinfo> | ||
|Lock Cassette - prevents transcription unless "unlocked" | |Lock Cassette - prevents transcription unless "unlocked" | ||
|Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) | |Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) | ||
| - | |[[ | + | |[[Media:BBa_K318030_pSB1C3.zip]] |
|} | |} | ||
<br> | <br> | ||
Revision as of 23:11, 26 October 2010
New Standards
Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:
- Genbank including features and plasmid (.gbk)
- Fwd and Reverse sequencing files (.ab1)
- Gels from cloning and confirmation (.pdf)
- All associated testing files and Results (.xls, .pdf, etc.)
Enzyme Delivery
New DNA
| Part Number | About | Testing in... | Zip File |
| <partinfo>BBa_k318512</partinfo> | Regulatory - pH inducible promoter | <partinfo>BBa_k318513</partinfo> | 512 |
| <partinfo>BBa_k318514</partinfo> | Gene - RamA protein can bind to cholic acid (bile salts) | <partinfo>BBa_k318515</partinfo> | File:Zip file ramA.zip |
| <partinfo>BBa_k318516</partinfo> | Regulatory - RamA/Bile-salt conjugate inducible promoter | <partinfo>BBa_k318517</partinfo> | File:Zip file acrRA.zip |
Testing Constructs
| Part Number | Function | Expression Type | Zip File |
| <partinfo>BBa_k318500</partinfo> | Produces Trascription Factor RcsB | Inducible - IPTG | 500 |
| <partinfo>BBa_k318501</partinfo> | Produces Trascription Factor RcsA | Inducible - IPTG | 501 |
| <partinfo>BBa_k318502</partinfo> | Produces Trascription Factor RcsA & RcsB | Inducible - IPTG | 502 |
| <partinfo>BBa_k318503</partinfo> | Produces Trascription Factor YgiV - Blocks Biofilm | Inducible - IPTG | 503 |
| <partinfo>BBa_k318504</partinfo> | Produces RfaI gene - WaaI Ligase | Inducible - IPTG | 504 |
| <partinfo>BBa_k318505</partinfo> | Produces T.F. YgiV & RfaI gene | Inducible - IPTG | 505 |
| <partinfo>BBa_k318506</partinfo> | Produces RFP | (+)LuxR, (-)cILVA | 506 |
| <partinfo>BBa_k318509</partinfo> | Produces Repressor cI-LVA (degredation tag) | Inducible - IPTG | 509 |
| <partinfo>BBa_k318510</partinfo> | Produces Repressor cI (no degredation tag) | Inducible - IPTG | 510 |
| <partinfo>BBa_k318511</partinfo> | Produces Activator LuxR | Anhydrous Tetrycycline | 511 |
| <partinfo>BBa_k318513</partinfo> | Produces RFP | pH | 513 |
| <partinfo>BBa_k318515</partinfo> | Produces RFP | RamA/Bile-Salt Complex | Media:Zip_file_acrRA.zip |
| <partinfo>BBa_k318517</partinfo> | Produces RamA gene | Bile Salt | Media:Zip_file_ramA.zip |
Final Constructs
| Part Number | Function | Expression Type |
| <partinfo>BBa_k3185##</partinfo> | Produces Trascription Factors RcsA & RcsB, and B-Gal | Constitutive |
| <partinfo>BBa_k3185##</partinfo> | A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) | pH 2 |
| <partinfo>BBa_k318507</partinfo> | A) Activates Lysis once in the small intestine | (+)-LuxR, (-)-cILVA |
| <partinfo>BBa_k318519</partinfo> | B) Activates lysis once in the small intestine | Bile Salts |
Encryption
| Part Number | Description | Notes | Zip File |
| <partinfo>BBa_K318000</partinfo> | Key Cassette - produces Cre and Hin recombinases | Arabinose induces Cre expression, IPTG induces Hin expression | Media:BBa_K318000_pSB1C3.zip |
| <partinfo>BBa_K318030</partinfo> | Lock Cassette - prevents transcription unless "unlocked" | Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) | Media:BBa_K318030_pSB1C3.zip |
Other Parts
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318010 BBa_K318010]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318011 BBa_K318011]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318012 BBa_K318012]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318013 BBa_K318013]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318014 BBa_K318014]
