Team:Wisconsin-Madison/results
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===Encapsulation: Cell Survivability in Acidic Conditions=== | ===Encapsulation: Cell Survivability in Acidic Conditions=== | ||
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===Timed Lysis: Inducible/Repressible Promoter=== | ===Timed Lysis: Inducible/Repressible Promoter=== | ||
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Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part | Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part | ||
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Revision as of 17:43, 26 October 2010
Enzyme Treatment
Encapsulation: Colonic Acid Quantification
Encapsulation: Cell Survivability in Acidic Conditions
Timed Lysis: Inducible/Repressible Promoter
gadAp: A pH Sensitive Promoter
Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part