Team:Wisconsin-Madison/results

From 2010.igem.org

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(Timed Lysis: Inducible/Repressible Promoter)
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==Enzyme Treatment==
==Enzyme Treatment==
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===Enzyme Production===
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===Encapsulation: Colonic Acid Quantification===
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===Encapsulation: Cell Survivability in Acidic Conditions===
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===Encapsulation and Acid Survivability===
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===Timed Lysis: Bile Promoter===
===Timed Lysis: Bile Promoter===

Revision as of 22:25, 23 October 2010

Enzyme Treatment

Encapsulation: Colonic Acid Quantification

Encapsulation: Cell Survivability in Acidic Conditions

Timed Lysis: Bile Promoter

Timed Lysis: Inducible/Repressible Promoter

gadAp: A pH Sensitive Promoter

Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part

Encryption