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Team:HKUST/future work
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<li><a href="https://2010.igem.org/Team:HKUST/Team">Here we are!</a></li> | <li><a href="https://2010.igem.org/Team:HKUST/Team">Here we are!</a></li> | ||
| - | + | <li><a>Project</a><li> | |
| - | + | <li class="project"><a href="https://2010.igem.org/Team:HKUST/Project">Abstract</a></li> | |
| - | + | <li class="project"><a href="https://2010.igem.org/Team:HKUST/Project/Background">Background</a></li> | |
<li class="project"><a href="https://2010.igem.org/Team:HKUST/Project/Experiment Design">Experiment Design</a></li> | <li class="project"><a href="https://2010.igem.org/Team:HKUST/Project/Experiment Design">Experiment Design</a></li> | ||
<li class="project"><a href="https://2010.igem.org/Team:HKUST/Project/Results and Discussion">Results and Discussion</a></li> | <li class="project"><a href="https://2010.igem.org/Team:HKUST/Project/Results and Discussion">Results and Discussion</a></li> | ||
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<li><a href="https://2010.igem.org/Team:HKUST/biobrick">Biobricks</a></li> | <li><a href="https://2010.igem.org/Team:HKUST/biobrick">Biobricks</a></li> | ||
| - | <li><a href="https://2010.igem.org/Team:HKUST/Human_Practice">Human Pratice</a></li> | + | <li><a href="https://2010.igem.org/Team:HKUST/Human_Practice">Human Pratice</a></li> |
| - | + | <li><a href="https://2010.igem.org/Team:HKUST/future_work">Future Work</a></li> | |
| + | <li><a href="https://2010.igem.org/Team:HKUST/Notebook">Lab Notebook</a></li> | ||
| + | <li><a href="https://2010.igem.org/Team:HKUST/attribution">Attribution and Contributions</a></li> | ||
<li><a href="https://2010.igem.org/Team:HKUST/Acknowledgement">Acknowledgement</a></li> | <li><a href="https://2010.igem.org/Team:HKUST/Acknowledgement">Acknowledgement</a></li> | ||
<li><a href="https://2010.igem.org/Team:HKUST/Safety">Biosafety</a></li> | <li><a href="https://2010.igem.org/Team:HKUST/Safety">Biosafety</a></li> | ||
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| + | <h2>Future Work for iGEM 2010 HKUST team</h2> | ||
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| + | <li>Transform <em>Lactobacillus plantarum</em> WCFS1 with our existing constructs [agrC-mCherry/agrC-plnB-mCherry in pMG36e]. Use fluorescence microscope to examine whether the chimeric AIP receptor localizes on <em>Lactobacillus</em> cell membrane.</li> | ||
| + | <br /> | ||
| + | <li>We will continue our "chimeric AIP receptor functionality testing" experiments in two<em> Lactobacillus</em> species,<em> L. plantarum</em> WCFS1 and<em> L. sakei</em> Lb790. We have already built the part [agrC/agrC-plnB in pMG36e] of our original design and we hope to complete the rest [plnA promoter – gusA report unit] soon. After obtaining the final construct [plnA promoter – gusA reporter – P32 promoter – agrC/agrC-plnB], we will transform the pMG36e shuttle vector into respective<em> Lactobacillus</em> cells. Finally, we will apply gusA reporter assay to determine whether the fusion AIP receptor functions as expected.</li> | ||
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| + | <li>Migrate the existing constructs "RIP synthesis and secretion cassette" [SP+DD13-RIP] from pBluescript to shuttle vector pMG36e.</li> | ||
| + | <br /> | ||
| + | <li>Perform functionality test to examine RIP secretion efficiency in <em>L. plantarum</em>.</li> | ||
| + | <br /> | ||
| + | <li>Conduct bio-assay of the reporter plasmid and estimate the inhibition efficiency of DD13-RIP against <em>S. aureus</em> growth.</li> | ||
| + | <br /> | ||
| + | <li>Combine constructs from two modules – "AIP Sensor on <em>Lactobacillus</em> Cell Membrane" and "RIP Synthesis and Secretion in <em>Lactobacillus</em>" – together. We will then transform the combined construct [plnA promoter – RIP synthesis and secretion cassette – P32 promoter – agrC/agrC-plnB] into <em>L. plantarum</em> and examine if our initial design works as expected.</li> | ||
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Revision as of 17:03, 23 October 2010
Future Work for iGEM 2010 HKUST team
- Transform Lactobacillus plantarum WCFS1 with our existing constructs [agrC-mCherry/agrC-plnB-mCherry in pMG36e]. Use fluorescence microscope to examine whether the chimeric AIP receptor localizes on Lactobacillus cell membrane.
- We will continue our "chimeric AIP receptor functionality testing" experiments in two Lactobacillus species, L. plantarum WCFS1 and L. sakei Lb790. We have already built the part [agrC/agrC-plnB in pMG36e] of our original design and we hope to complete the rest [plnA promoter – gusA report unit] soon. After obtaining the final construct [plnA promoter – gusA reporter – P32 promoter – agrC/agrC-plnB], we will transform the pMG36e shuttle vector into respective Lactobacillus cells. Finally, we will apply gusA reporter assay to determine whether the fusion AIP receptor functions as expected.
- Migrate the existing constructs "RIP synthesis and secretion cassette" [SP+DD13-RIP] from pBluescript to shuttle vector pMG36e.
- Perform functionality test to examine RIP secretion efficiency in L. plantarum.
- Conduct bio-assay of the reporter plasmid and estimate the inhibition efficiency of DD13-RIP against S. aureus growth.
- Combine constructs from two modules – "AIP Sensor on Lactobacillus Cell Membrane" and "RIP Synthesis and Secretion in Lactobacillus" – together. We will then transform the combined construct [plnA promoter – RIP synthesis and secretion cassette – P32 promoter – agrC/agrC-plnB] into L. plantarum and examine if our initial design works as expected.
