Team:Imperial College London/Lab Diaries/Vectors team

From 2010.igem.org

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We are assembling the AmyE and PyrD vectors in order to transform B. Subtilis with our parts. Once completed, these vectors will be reusable and can then be used to introduce any relevant piece of DNA directly into B.subtilis genome. The vectors will be used both for the final assembly and for testing constructs.
We are assembling the AmyE and PyrD vectors in order to transform B. Subtilis with our parts. Once completed, these vectors will be reusable and can then be used to introduce any relevant piece of DNA directly into B.subtilis genome. The vectors will be used both for the final assembly and for testing constructs.
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*[[IGEM:Imperial/2010/PyrD_Vector | Assembly of the<font color = #6B3FA0> '''PyrD vector''' </font>]]
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==PyrD Vector==
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*[[IGEM:Imperial/2010/AmyE_Vector | Assembly of the<font color = #CA2C92> '''AmyE vector'''</font>]]
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*[[IGEM:Imperial/2010/PyrD_Vector | Assembly of the<font color = #6B3FA0> '''PyrD vector Assembly''' </font>]]
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==Assembly of Vectors==
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[[Image:IC_PyrD.JPG |750px|thumb|center|alt=A|Overveiw of PyrD assembly]]
[[Image:IC_PyrD.JPG |750px|thumb|center|alt=A|Overveiw of PyrD assembly]]
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==AmyE Vector==
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*[[IGEM:Imperial/2010/AmyE_Vector | Assembly of the<font color = #CA2C92> '''AmyE vector Assembly'''</font>]]
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[[Image:IC_AmyE.JPG |750px|thumb|center|alt=A|Overveiw of AmyE assembly]]
[[Image:IC_AmyE.JPG |750px|thumb|center|alt=A|Overveiw of AmyE assembly]]

Revision as of 18:35, 23 October 2010

Lab Diaries Overview | Surface Protein Team | XylE Team | Vectors Team | Modelling Team
Here are the technical diaries for our project. We've split them up into three lab teams and the modelling team. We think it's really important that absolutely anyone can find out what we've been doing. For a really detailed look at what we did, and when, you've come to the right place!
Imperial iGEM 2010

Follow our progress: Click me!

Objectives

We are assembling the AmyE and PyrD vectors in order to transform B. Subtilis with our parts. Once completed, these vectors will be reusable and can then be used to introduce any relevant piece of DNA directly into B.subtilis genome. The vectors will be used both for the final assembly and for testing constructs.

PyrD Vector

A
Overveiw of PyrD assembly

AmyE Vector

A
Overveiw of AmyE assembly