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Team:Osaka/Protocols
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*Preparation of competent cells for heat-shock transformation | *Preparation of competent cells for heat-shock transformation | ||
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*Transformation by heat-shock | *Transformation by heat-shock | ||
*Plasmid DNA miniprep | *Plasmid DNA miniprep | ||
Revision as of 06:59, 24 October 2010
Protocols
- Preparation of competent cells for heat-shock transformation
- Preparation
- Transformation by heat-shock
- Plasmid DNA miniprep
- 3A BioBrick assembly
- PCR cloning
- PCR site-directed mutagenesis
- Extraction of genome DNA for PCR cloning
