From 2010.igem.org
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| | || Part || 3OC6-HSL concentration [uM] | | || Part || 3OC6-HSL concentration [uM] |
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| - | || || | + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.4 |
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| - | || || | + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.02 |
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| - | || || | + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.1 |
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| - | || || | + | || <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> || ~0.002 |
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| - | || || | + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || ~0.005 |
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| - | || || | + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || ~0.002 |
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| - | || || | + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || ~0.003 |
| | |} | | |} |
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| | + | Concentrations of 3OC6-HSL were obtained multiplying the values read on the graphics for the corresponding dilution factor. |
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| | <!-- table previous next test --> | | <!-- table previous next test --> |
Revision as of 10:24, 23 October 2010
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SEPTEMBER, 10TH
PLATE
EXPERIMENT DESCRIPTION
Motivation
The experiment was performed to measure the 3OC6-HSL concentration due to the synthesis of homoserine-lactone by 3OC6-HSL generators in saturation phase of the cultures growth.
Methods
According to Protocol 3 the experiment was performed in LB on the following signalling parts:
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18)
- <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name: I14-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name: I15-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name: I16-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name: I17-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name: I18-4C5)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name: I19-4C5)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0031</partinfo> (negative control)
- <partinfo>pSB1AK3</partinfo> - <partinfo>BBa_K300009</partinfo> (negative control)
Supernatants were properly diluted in order to evaluate the production of 3OC6-HSL of the low copy number parts.
RESULTS
The 3OC6-HSL concentrations of several cultures were evaluated:
| Part | 3OC6-HSL concentration [uM]
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| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300030</partinfo> | ~0.4
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| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300028</partinfo> | ~0.02
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| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300025</partinfo> | ~0.1
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| <partinfo>BBa_J61002</partinfo> - <partinfo>BBa_K300027</partinfo> | ~0.002
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> | ~0.005
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> | ~0.002
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| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> | ~0.003
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Concentrations of 3OC6-HSL were obtained multiplying the values read on the graphics for the corresponding dilution factor.
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