Team:Michigan/Project
From 2010.igem.org
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=== Quorum Sensing === | === Quorum Sensing === | ||
- | Our cells will use quorum sensing to determine when the flocculation will start. | + | Our cells will use quorum sensing to determine when the flocculation will start. The cells will produce an inducer either AHL or AI-2. |
=== Pili Hyperexpression === | === Pili Hyperexpression === | ||
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Type 1 pili are proteinaceous adhesins that are found on the surface of E. coli. The pili help E. coli. form biofilms. By overproducing the pili, we hope to increase flocculation. Another gene we are looking at controls pilus adhesin, which is an adhesive substance located on the pili. By hyperexpressing this gene, we can increase the amount of pilus adhesin (Fig. 1), which will hopefully strengthen the E. coli binding to the algae. | Type 1 pili are proteinaceous adhesins that are found on the surface of E. coli. The pili help E. coli. form biofilms. By overproducing the pili, we hope to increase flocculation. Another gene we are looking at controls pilus adhesin, which is an adhesive substance located on the pili. By hyperexpressing this gene, we can increase the amount of pilus adhesin (Fig. 1), which will hopefully strengthen the E. coli binding to the algae. | ||
[[Image:Michigan-PilusAdhesin.jpg|300px|thumb|Fig. 1 C shows normal pili, B shows hyperexpressed pilus adhesin.]] | [[Image:Michigan-PilusAdhesin.jpg|300px|thumb|Fig. 1 C shows normal pili, B shows hyperexpressed pilus adhesin.]] | ||
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=== Virus Surface Display === | === Virus Surface Display === | ||
=== Washboard === | === Washboard === |
Revision as of 01:25, 28 June 2010