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Team:TU Delft/22 June 2010 content
From 2010.igem.org
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Revision as of 12:59, 24 June 2010
We tried to compensate for yesterdays delays. We transformed 15 Biobricks from the distribution plates!
Some PCR reactions were performed on short BioBrick inserts which would eventually save time in the long-run (compared to transforming and plasmid isolation). The BioBricks involved were J61100, J61101, J61107, J61117, J61127 and B0034.
The protocol was as follows:
| - | uL |
| Master Mix | 25 |
| BB template (10x diluted) | 1 |
| Primers G00100 and G00101 | 3 |
| H20 | 18
} |
