Team:USTC/Project/safe

From 2010.igem.org

(Difference between revisions)
(→experimental safety)
(→experimental safety)
Line 9: Line 9:
== experimental safety ==
== experimental safety ==
-
*From Feb.4th to Feb.13th, a collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. Also,it`s the time we get to know each other and become a team as a whole.
+
*Just like any other practical activities in school science, all microbiology investigations require the user to adopt good laboratory practice. The [[Team:USTC/Notebook| preliminary experiment training]] made sure that every one of us get familiar with the whole system of basic microbiology investigations and get adroit skills especially for those in related majors.
 +
*'''Several regulations has been set to guarantee personal protection.''' <br />
 +
<!--[http://www.microbiologyonline.org.uk/safety.html| refer to messages online]
 +
-->
 +
**Food or drink should not be stored or consumed in a laboratory or prep room that is used for microbiology. <br />
 +
**No-one should lick labels, apply cosmetics, chew gum, suck pens or pencils or smoke in a laboratory or prep room. <br />
 +
**Facilities should be provided within the laboratory or prep room for hands to be washed with soap after handling microbial cultures and whenever leaving the lab. <br />
 +
**Paper towels or some other hygienic method should be used for drying hands. If contamination of the hands is suspected, then they should be washed immediately with soap and water. <br />
 +
**Cuts or abrasions should be protected by the use of waterproof dressings or by wearing disposable gloves. <br />
 +
**Safety glasses should be worn in some certain circumstances,eg. when using a UV lamp.<br />
-
**The contents of the training are listed as followed:
+
*'''Work Safety in our lab is categorized into two levels as follows:'''
-
***1. outlines
+
**Level 1: ''organisms we work with have little known risk and are observed in the closed containers in which they are grown.'' Microbes ('''K12''') we growing is tested as non-risk and harmless, furthermore, by culturing closed containers which are taped before examination and remain unopened unless the cultures inside have been killed by the autoclave sterilizer. We made every effort to exclude any possibility of unsafety.  
-
***2. E.coli culture (Medium preparation, Inoculation, Aseptic operation)
+
**Level 2: ''biological reagents we use are considered on environmental and human safety.'' We choose '''GelRed'''[http://www.biotium.com/product/price_and_info.asp?item=41001&Sub_Section=09A] instead of EB as our nuleic acid stains.
-
***3. plasmid extraction
+
-
***4. PCR
+
-
***5. DNA Electrophoresis
+
-
***6. Double Digest
+
-
***7. reclaim and appraise of the product of enzyme cutting
+
-
***8. Ligation
+
-
***9. preparation of competent cell
+
-
***10.  genomic screening of masculine clone
+
-
***11. DNA sequencing
+
== asking the experts ==
== asking the experts ==

Revision as of 12:12, 21 October 2010

An Integrated Platform Based on Bacterial Microcompartment for de novo Proteinaceous Artificial Organelles


overview

experimental safety

  • Just like any other practical activities in school science, all microbiology investigations require the user to adopt good laboratory practice. The preliminary experiment training made sure that every one of us get familiar with the whole system of basic microbiology investigations and get adroit skills especially for those in related majors.
  • Several regulations has been set to guarantee personal protection.
    • Food or drink should not be stored or consumed in a laboratory or prep room that is used for microbiology.
    • No-one should lick labels, apply cosmetics, chew gum, suck pens or pencils or smoke in a laboratory or prep room.
    • Facilities should be provided within the laboratory or prep room for hands to be washed with soap after handling microbial cultures and whenever leaving the lab.
    • Paper towels or some other hygienic method should be used for drying hands. If contamination of the hands is suspected, then they should be washed immediately with soap and water.
    • Cuts or abrasions should be protected by the use of waterproof dressings or by wearing disposable gloves.
    • Safety glasses should be worn in some certain circumstances,eg. when using a UV lamp.
  • Work Safety in our lab is categorized into two levels as follows:
    • Level 1: organisms we work with have little known risk and are observed in the closed containers in which they are grown. Microbes (K12) we growing is tested as non-risk and harmless, furthermore, by culturing closed containers which are taped before examination and remain unopened unless the cultures inside have been killed by the autoclave sterilizer. We made every effort to exclude any possibility of unsafety.
    • Level 2: biological reagents we use are considered on environmental and human safety. We choose GelRed[http://www.biotium.com/product/price_and_info.asp?item=41001&Sub_Section=09A] instead of EB as our nuleic acid stains.

asking the experts

answering key questions

For iGEM 2010 teams are asked to detail how they approached any issues of biological safety associated with their projects. Specifically, teams should consider the following questions:

  1. Would any of your project ideas raise safety issues in terms of:
    • researcher safety,
    • public safety, or
    • environmental safety?
  2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
    • did you document these issues in the Registry?
    • how did you manage to handle the safety issue?
    • How could other teams learn from your experience?
  3. Is there a local biosafety group, committee, or review board at your institution?
    • If yes, what does your local biosafety group think about your project?
    • If no, which specific biosafety rules or guidelines do you have to consider in your country?
  4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

 
Retrieved from "http://2010.igem.org/Team:USTC/Project/safe"