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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14
From 2010.igem.org
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<Sample><br /> | <Sample><br /> | ||
*vector | *vector | ||
| - | *9-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9-7] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | + | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ] | ||
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<Result><br /> | <Result><br /> | ||
[[Image:Matsuura_2010-10-14_22hr_56min.JPG]] | [[Image:Matsuura_2010-10-14_22hr_56min.JPG]] | ||
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===11：DNA extraction from gels=== | ===11：DNA extraction from gels=== | ||
Revision as of 12:33, 19 October 2010
Contents |
2010/10/14
1:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
2：DNA extraction
＜Member＞
nito
＜Sample＞
＜Protocol＞
See Protocol 4
3：DNA Ligation
＜Member＞
nito
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
4：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
- plasmid
・Digest enzyme
- XbaⅠ
- SpeⅠ
＜Protocol＞
See Prptpcpl 9
5:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 1
6：DNA extraction from gels
＜Member＞
nito
＜Sample＞
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
7：DNA Digestion
＜Member＞
nito
＜Sample, Materials＞
・PCR productions
・Digest enzyme
- AvrⅡ
- NheⅠ
- XbaⅠ
- SpeⅠ
＜Protocol＞
See Prptpcpl 9
8:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 1
9：DNA extraction from gels
＜Member＞
nito
＜Sample＞
- 9-7
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
10:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
<Result>
11：DNA extraction from gels
＜Member＞
nito
＜Sample＞
- 9-7
- 4-5
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
