Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14

From 2010.igem.org

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Revision as of 12:32, 19 October 2010

Contents

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-10-14 14hr 29min.JPG

2:DNA extraction

<Member>
nito

<Sample>

<Protocol>
See Protocol 4

3:DNA Ligation

<Member>
nito

<Sample>

(after digestion)

<Protocol>
See Protocol 10

4:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

  • plasmid

・Digest enzyme

  • XbaⅠ
  • SpeⅠ

<Protocol>
See Prptpcpl 9

5:PCR

<Member>
nito

<Sample>


<Protocol>
See Protocol 1

6:DNA extraction from gels

<Member>
nito

<Sample>

  • 9-7

(After electrophoresis)


<Protocol>
SeeProtocol 4

7:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

  • 1-2
  • 1-2-7
  • 10-2-7

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • XbaⅠ
  • SpeⅠ

<Protocol>
See Prptpcpl 9

8:PCR

<Member>
nito

<Sample>

  • 9
  • 9-7


<Protocol>
See Protocol 1

9:DNA extraction from gels

<Member>
nito

<Sample>

  • 9-7

(After electrophoresis)


<Protocol>
SeeProtocol 4

10:Electrophoresis

<Member>
nito

<Sample>

  • vector
  • 9-7
  • 4-5


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-10-14 22hr 56min.JPG


11:DNA extraction from gels

<Member>
nito

<Sample>

  • 9-7
  • 4-5

(After electrophoresis)


<Protocol>
SeeProtocol 4