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Team:Harvard/parts
From 2010.igem.org
(Difference between revisions)
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<tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr> | <tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr> | ||
| - | <tr><td>F1</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td></td><td></td><td></td></tr> | + | <tr><td>F1</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>F2</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td></td><td></td><td></td></tr> | + | <tr><td>F2</td><td>NOSt terminator</td><td>Plant specific terminator. Terminates transcription but no stop codon on end.</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>F3</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on end.</td><td></td><td></td><td></td></tr> | + | <tr><td>F3</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on end.</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>F4</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td></td><td></td><td></td></tr> | + | <tr><td>F4</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>F5</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td></td><td></td><td></td></tr> | + | <tr><td>F5</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>F6</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td></td><td></td><td></td></tr> | + | <tr><td>F6</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>F7</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td></td><td></td><td></td></tr> | + | <tr><td>F7</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>F8</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td></td><td></td><td></td></tr> | + | <tr><td>F8</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>F9</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td></td><td></td><td></td></tr> | + | <tr><td>F9</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K382010</td><td>amiRNA LUT2</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K382011</td><td>amiRNA BETA-OHASE 1</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
| - | <tr><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td></td><td></td><td></td></tr> | + | <tr><td>BBa_K382012</td><td>amiRNA LYC</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>chloramphenicol</td><td>-</td><td></td></tr> |
<tr><td>GF1</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td>-</td><td></td></tr> | <tr><td>GF1</td><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td>-</td><td></td></tr> | ||
<tr><td>GF2</td><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td>-</td><td></td></tr> | <tr><td>GF2</td><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td>-</td><td></td></tr> | ||
| - | <tr><td>GF3</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td></td></tr> | + | <tr><td>GF3</td><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td></td> </tr> |
<tr><td>GF4</td><td>RXRLc-VP16</td><td> Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td></td></tr> | <tr><td>GF4</td><td>RXRLc-VP16</td><td> Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td></td></tr> | ||
<tr><td>GF5</td><td>EcR-Gal4</td><td> Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td></td></tr> | <tr><td>GF5</td><td>EcR-Gal4</td><td> Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td></td></tr> | ||
</table> | </table> | ||
<br /><br /> | <br /><br /> | ||
Revision as of 05:47, 19 October 2010
parts and primers
| Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence |
| V1 | pORE Open Series 1 (O1) | Agrobacterium vector open series | Kanamycin | pat | |
| V2 | pORE Open Series 2 (O2) | Agrobacterium vector open series | Kanamycin | nptII | |
| V3 | pORE Expression Series 3 (E3) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | pat | |
| V4 | pORE Expression Series 4 (E4) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | nptII | |
| V5 | pORE Reporter Series 1 (R1) | Agrobacterium vector with gusA reporter | Kanamycin | nptII | |
| V6 | pORE Reporter Series 3 (R3) | Agrobacterium vector with smgfp reporter | Kanamycin | nptII | |
| V7 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | pat | ||
| V8 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | nptII | ||
| V9 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | pat | ||
| V10 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | nptII | ||
| V11 | Agrobacterium vector with gusA reporter and biobrick cloning site | Kanamycin | nptII | ||
| V12 | Agrobacterium vector with smgfp reporter and biobrick cloning site | Kanamycin | nptII | ||
| A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | - | amirnaGFP |
| A2 | amiRNA LTP | LTP knockdown construct | chloramphenicol | - | amiRNA LTP |
| A3 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | - | ihpRNA Bet1 |
| A4 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | - | ihpRNA LTP |
| A5 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | - | ihpRNA GER |
| F1 | pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | chloramphenicol | - | |
| F2 | NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | chloramphenicol | - | |
| F3 | NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on end. | chloramphenicol | - | |
| F4 | Brazzein | Sweet-tasting protein. 54 amino acid sequence. | chloramphenicol | - | |
| F5 | Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | chloramphenicol | - | |
| F6 | Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | |
| F7 | Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | |
| F8 | Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | |
| F9 | Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | |
| BBa_K382010 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown | chloramphenicol | - | |
| BBa_K382011 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | chloramphenicol | - | |
| BBa_K382012 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | chloramphenicol | - | |
| GF1 | Barnase | Fatal gene for the genetic fence | chloramphenicol | - | |
| GF2 | Barstar | Protein inhibitor of Barnase | chloramphenicol | - | |
| GF3 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | ||
| GF4 | RXRLc-VP16 | Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide | chloramphenicol | - | |
| GF5 | EcR-Gal4 | Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide | chloramphenicol | - |
