Team:Harvard/parts

From 2010.igem.org

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<tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td></td><td></td><td></td><td></td></tr>
<tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td></td><td></td><td></td><td></td></tr>
<tr><td>BBa_K382012</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td></td><td></td><td></td><td></td></tr>
<tr><td>BBa_K382012</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td></td><td></td><td></td><td></td></tr>
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<tr><td>Barnase</td><td> Fatal gene for the genetic fence </td><td>chloramphenicol</td><td>-</td><td></td></tr>
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<tr><td>Barstar</td><td>Protein inhibitor of Barnase</td><td>chloramphenicol</td><td>-</td><td></td></tr>
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<tr><td>LacIN</td><td>Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter</td><td>chloramphenicol</td><td></td></tr>
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<tr><td>RXRLc-VP16</td><td> Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide</td><td>chloramphenicol</td><td>-</td><td></td></tr>
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<tr><td>EcR-Gal4</td><td> Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide </td><td>chloramphenicol</td><td>-</td><td></td></tr>
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Revision as of 05:38, 19 October 2010

parts and primers

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpat
V2pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptII
V3pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpat
V4pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptII
V5pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptII
V6pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptII
V7
-
Agrobacterium vector open series with biobrick cloning siteKanamycinpat
V8
-
Agrobacterium vector open series with biobrick cloning siteKanamycinnptII
V9
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpat
V10
-
Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptII
V11
-
Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptII
V12
-
Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptII
A1amiRNA GFPGFP knockdown constructchloramphenicol- amirnaGFP
A2amiRNA LTP LTP knockdown constructchloramphenicol- amiRNA LTP
A3ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol- ihpRNA Bet1
A4ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicol-ihpRNA LTP
A5ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicol-ihpRNA GER
F1pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants.
F2NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.
F3NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on end.
F4BrazzeinSweet-tasting protein. 54 amino acid sequence.
F5MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequence
F6Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression
F7Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression
F8Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.
F9Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.
BBa_K382010amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown
BBa_K382011amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown
BBa_K382012amiRNA construct for lycopene beta cyclase (LYC) knockdown
Barnase Fatal gene for the genetic fence chloramphenicol-
BarstarProtein inhibitor of Barnasechloramphenicol-
LacINLac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoterchloramphenicol
RXRLc-VP16 Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozidechloramphenicol-
EcR-Gal4 Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide chloramphenicol-


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