"
Talk:Team:IvyTech-South Bend/8 October 2010
From 2010.igem.org
(Difference between revisions)
Rchamberlin (Talk | contribs) (New page: == 10/8/10 == Making 4 1L bottlse of LB-Lennox Broth 2- with Agar 2- without I placed between 10.035 - 10.005 grams into each of the 4 bottles, then added 500 ml 18 ohm H2O into each...) |
Rchamberlin (Talk | contribs) |
||
| Line 1: | Line 1: | ||
| - | |||
== 10/8/10 == | == 10/8/10 == | ||
| Line 19: | Line 18: | ||
After done , cool to room temp label and place into fridge until needed. | After done , cool to room temp label and place into fridge until needed. | ||
| + | |||
| + | |||
| + | == 1:00 == | ||
Revision as of 20:35, 17 October 2010
10/8/10
Making 4 1L bottlse of LB-Lennox Broth
2- with Agar 2- without
I placed between 10.035 - 10.005 grams into each of the 4 bottles, then added 500 ml 18 ohm H2O into each bottle
Placed into the microwave on beverage setting 8oz.
after first time removed the 2 -just broth
heated the 2- with Agar again at the same setting.
Added to 2- with Agar - 7.002 grams to each then placed into the autoclave on liquid setting/ 121 C for 15 min.
After done , cool to room temp label and place into fridge until needed.
