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Talk:Team:IvyTech-South Bend/5 October 2010
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After waiting 45 min, I streaked on an Amp/Agar plate and placed into incubator @ 37C overnight placing the rest of the spl. in incubator @ 37C. | After waiting 45 min, I streaked on an Amp/Agar plate and placed into incubator @ 37C overnight placing the rest of the spl. in incubator @ 37C. | ||
| - | --JHull 5 October 2010 [[User:Rchamberlin|Rchamberlin]] 20:10, 17 October 2010 (UTC) | + | --for JHull 5 October 2010 [[User:Rchamberlin|Rchamberlin]] 20:10, 17 October 2010 (UTC) |
| + | ==10/5/10== | ||
| + | Suspension of A.tumefaciens and A.T9002 in LB amp | ||
| + | |||
| + | I’m suspending Agro Tumefaciens +T9002 in 20 ml of LB/Broth w/ 20 ul of Amp. in the A.T9002 suspension only | ||
| + | 1- first I poured 20 ml of LB Broth (prepared by JH) into 6 - .45 ml steril tubes | ||
| + | |||
| + | 2- I then added 20 ul of Amp into the tube, (only one containing Agro T9002) I pulled a sample of Agro + T9002 from a colony and suspended it into my LB/Amp Broth (plate streaked by IF) | ||
| + | |||
| + | 3- I pulled cells from the colony A.Tume. and suspended them in LB Broth (20 ml) | ||
| + | |||
| + | 4- Next, I pulled cells from a colony B.Thurlgensis (made by AM) and suspended in 20 ml of LB Broth | ||
| + | |||
| + | 5- I pulled cells from plate containing B. Magetarium and suspended in Broth. | ||
| + | |||
| + | --for DGarvey 5 October 2010 [[User:Rchamberlin|Rchamberlin]] 16:41, 27 October 2010 (UTC) | ||
Revision as of 16:41, 27 October 2010
Contents |
10/5/2010
pTet GFP
Untagged GFP behind a constitutive promoter. Usage and Biology
GFP only control Spring 2010 Distribution
2010 Kit Plate 2 8A pSB1A2
Part:BBa_I732017
Extracting DNA from I732017 using protocol from p 19 after extraction placing in freezer.
I13511
H.Y Electroporated a part I13511 with E-Coli,
After waiting 45 min, I streaked on an Amp/Agar plate and placed into incubator @ 37C overnight placing the rest of the spl. in incubator @ 37C.
--for JHull 5 October 2010 Rchamberlin 20:10, 17 October 2010 (UTC)
10/5/10
Suspension of A.tumefaciens and A.T9002 in LB amp
I’m suspending Agro Tumefaciens +T9002 in 20 ml of LB/Broth w/ 20 ul of Amp. in the A.T9002 suspension only 1- first I poured 20 ml of LB Broth (prepared by JH) into 6 - .45 ml steril tubes
2- I then added 20 ul of Amp into the tube, (only one containing Agro T9002) I pulled a sample of Agro + T9002 from a colony and suspended it into my LB/Amp Broth (plate streaked by IF)
3- I pulled cells from the colony A.Tume. and suspended them in LB Broth (20 ml)
4- Next, I pulled cells from a colony B.Thurlgensis (made by AM) and suspended in 20 ml of LB Broth
5- I pulled cells from plate containing B. Magetarium and suspended in Broth.
--for DGarvey 5 October 2010 Rchamberlin 16:41, 27 October 2010 (UTC)
