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               <b><i>Theoretical Results </b></i></font>  
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Six promoters were observed to show enhanced expression during exposure to light conditions compared to darkness. Of these six, psbA2 showed the highest level of light induction. These six promoters did not show significant downregulation during exposure to darkness, with the exception of sigE, which appears to be linked to circadian rhythm. Only one promoter, lrtA, was observed to show increased expression during the dark treatment. All expression levels were relative to sigA promoter expression, which is not altered under any cellular or environmental condition.
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Two promoters were observed to have increased expression and no promoters were observed to be downregulated during heat treatment. The hspA promoter had greatly enhanced transcription, and the sigB promoter was observed to have slightly increased transcription. The lack of heat-inactivated promoters is likely due to the small number of total promoters tested, rather than the absence of these promoters in PCC 6803.
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Two promoters were observed to have increased expression and no promoters were observed to be downregulated during nitrogen-stress. The pglnBP2 promoter, which was induced, naturally procedures a gene that plays some, yet to be determined, role in the nitrogen response. The sigB promoter was also observed to increase its level of transcription, making it the only promoter in this study that was induced by more than one condition. This is likely due to the fact that it regulates the SigB protein, which is the general stress response sigma factor. Once again, like the heat treatment, no promoter was observed to be downregulated, likely due to the limited set of tested promoters.
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Five promoters were not observed to change expression levels under any treatment condition, and were deemed constitutive. These five promoters induce transcription at five separate levels, from undetectable levels with sigF to normal protein levels. These promoters may not be constitutive for all conditions, but may be induced during other conditions not tested in this study – the most notable of which is osmotic stress.
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Revision as of 06:28, 17 October 2010

Utah State University

CyanoBricks
Theoretical Results

Six promoters were observed to show enhanced expression during exposure to light conditions compared to darkness. Of these six, psbA2 showed the highest level of light induction. These six promoters did not show significant downregulation during exposure to darkness, with the exception of sigE, which appears to be linked to circadian rhythm. Only one promoter, lrtA, was observed to show increased expression during the dark treatment. All expression levels were relative to sigA promoter expression, which is not altered under any cellular or environmental condition.

Two promoters were observed to have increased expression and no promoters were observed to be downregulated during heat treatment. The hspA promoter had greatly enhanced transcription, and the sigB promoter was observed to have slightly increased transcription. The lack of heat-inactivated promoters is likely due to the small number of total promoters tested, rather than the absence of these promoters in PCC 6803.

Two promoters were observed to have increased expression and no promoters were observed to be downregulated during nitrogen-stress. The pglnBP2 promoter, which was induced, naturally procedures a gene that plays some, yet to be determined, role in the nitrogen response. The sigB promoter was also observed to increase its level of transcription, making it the only promoter in this study that was induced by more than one condition. This is likely due to the fact that it regulates the SigB protein, which is the general stress response sigma factor. Once again, like the heat treatment, no promoter was observed to be downregulated, likely due to the limited set of tested promoters.

Five promoters were not observed to change expression levels under any treatment condition, and were deemed constitutive. These five promoters induce transcription at five separate levels, from undetectable levels with sigF to normal protein levels. These promoters may not be constitutive for all conditions, but may be induced during other conditions not tested in this study – the most notable of which is osmotic stress.



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