"

From 2010.igem.org

Revision as of 23:07, 16 October 2010 (view source) Gcxmatt (Talk | contribs) ← Older edit		Revision as of 02:10, 27 October 2010 (view source) Gcxmatt (Talk | contribs) (→9/7/10) Newer edit →
Line 7:		Line 7:
	===9/7/10===		===9/7/10===
-	********	+	Day Off
	----		----
		+
	===9/8/10===		===9/8/10===
	********		********

---

Revision as of 02:10, 27 October 2010

Week13 Highlights

We began reorganizing our lab space and equipment in preparation for a fall quarter class that would be utilizing the majority of the lab. We attempted standard assembly of LacP-RBS-CHS3 because the presence of a P site in CHS3 prevented 3A assembly.

---

9/6/10

Kevin did some reorganizing to get the lab back in order for Prof. Mordacq's class this fall. He consolidated the bench space, the -20 refrigerator and the cold room.

9/7/10

Day Off

---

9/8/10

---

9/9/10

Assembled LacP-RBS1-CHS3 in a Chlor backbone plasmid using standard assembly methods Cut LacP-RBS1 using S Cut CHS3 using X and S (50% chance of inserting backwards --> must select for this after)

---

9/10/10

Inoculated 20 LacP-RBS-CHS3 cells.

Assembled CP-LacPI-RFP parts into Tet backbone.

---