Team:Northwestern/Project/Chassis

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Our team primarily used the high transformation efficiency top10 cells from invitrogen for most protocols.

In order to ensure lawn formation, the TqsA (also known as the YdgG) knockout were taken from a copy of the Keio Collection with permission from Professor Margaret Saks of Northwestern University. The ΔTqsA strain increases the thickness of biofilm by interfering with the quorum sensing mechanism (more specifically, the autoinducer-2 transport system).

To prevent bacteria from digesting synthesized Chitin, as it normally would, the ΔChiA strain was also taken from the Keio Collection. Because this strain lacks Chitinase, a digestive enzyme that breaks the glycosidic bonds in Chitin.

Invitrogen Top10 Cells:

http://products.invitrogen.com/ivgn/product/C404010

TqsA aka YdgG Knockouts:

http://jb.asm.org/cgi/content/short/188/2/587

http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW1593

http://cgsc2.biology.yale.edu/Mutation.php?ID=106356

ChiA Knockouts:

http://cgsc2.biology.yale.edu/Mutation.php?ID=100308

http://www.ncbi.nlm.nih.gov/gene/947837

http://ecoli.aist-nara.ac.jp/GB5/info.jsp?id=JW3300

MAGE Protocol

http://www.wired.com/wiredscience/2009/07/cellfactories/

http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html

Revision as of 19:13, 16 October 2010 (view source) Cyu286 (Talk \| contribs) ← Older edit		Revision as of 19:14, 16 October 2010 (view source) Cyu286 (Talk \| contribs) Newer edit →
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	To prevent bacteria from digesting synthesized Chitin, as it normally would, the ΔChiA strain was also taken from the Keio Collection. Because this strain lacks Chitinase, a digestive enzyme that breaks the glycosidic bonds in Chitin.		To prevent bacteria from digesting synthesized Chitin, as it normally would, the ΔChiA strain was also taken from the Keio Collection. Because this strain lacks Chitinase, a digestive enzyme that breaks the glycosidic bonds in Chitin.
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	''Invitrogen Top10 Cells:''		''Invitrogen Top10 Cells:''