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From 2010.igem.org

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	===3：DNA extraction from gels===		===3：DNA extraction from gels===

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Revision as of 11:29, 16 October 2010

Contents 1 2010/10/15 1.1 1：DNA extraction 1.2 2:Electrophoresis 1.3 3：DNA extraction from gels 1.4 4:PCR 1.5 5:Electrophoresis 1.6 6:PCR 1.7 7：DNA Digestion 1.8 8：DNA Ligation 1.9 9:PCR 1.10 10:PCR 1.11 11:Electrophoresis 1.12 12:Transformation 1.13 13:Electrophoresis 1.14 14:Electrophoresis

2010/10/15

1：DNA extraction

＜Member＞
Hitomi

＜Sample＞

• 4-5
• 9-7
• vector

＜Protocol＞
SeeProtocol 4

2:Electrophoresis

<Member>
Hitomi

<Sample>

• 4-5
• 9-7

<Protocol>
See Protocol 8

<Result>

3：DNA extraction from gels

＜Member＞
Mariko

＜Sample＞

• 1+2(8/31)
• 4+5(8/31)

(After electrophoresis)

＜Protocol＞
SeeProtocol 4

4:PCR

<Member>
hito

<Sample>(temperature in annealing:℃)

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• 1-2
• 4-5
• 7-8-10

<Protocol>
See Protocol 2

5:Electrophoresis

<Member>
mio,mizuki

<Sample>
PCR products (9/16)

<Protocol>
See Protocol 8

<Result>

6:PCR

<Member>
hito

<Sample>(temperature in annealing:℃)

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• 1-2
• 4-5
• 7-8-10

<Protocol>
See Protocol 2

7：DNA Digestion

＜Member＞

＜Sample, Materials＞
・PCR productions

• 1L(8/25) 8μl
• 2L(8/26) 8μl
• 4H(8/26) 16μl
• 5H(8/26) 16μl

・Digest enzyme

• AvrⅡ
• NheⅠ
• SpeⅠ

＜Protocol＞
See Protocol 9

8：DNA Ligation

＜Member＞

＜Sample＞

• 1L(8/25)
• 2L(8/26)
• 4H(8/26)
• 5H(8/26)

(after digestion)

＜Protocol＞
See Protocol 3

9:PCR

<Member>
hito

<Sample>(temperature in annealing:℃)

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• 1-2
• 4-5
• 7-8-10

<Protocol>
See Protocol 2

10:PCR

<Member>
hito

<Sample>(temperature in annealing:℃)

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• 1-2
• 4-5
• 7-8-10

<Protocol>
See Protocol 2

11:Electrophoresis

<Member>
mio,mizuki

<Sample>
PCR products (9/16)

<Protocol>
See Protocol 8

<Result>

12:Transformation

<Member>
nito

<Sample>

• 7-8-10
• JM109

<Protocol>
See Protocol 7

13:Electrophoresis

<Member>
mio,mizuki

<Sample>
PCR products (9/16)

<Protocol>
See Protocol 8

<Result>

14:Electrophoresis

<Member>
mio,mizuki

<Sample>
PCR products (9/16)

<Protocol>
See Protocol 8

<Result>