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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15
From 2010.igem.org
(New page: =2010/10/15= ===1：DNA extraction=== ＜Member＞<br /> Hitomi ＜Sample＞<br /> ・PCR productions (8/31) ＜Protocol＞<br /> See[https://2010.igem.org/Team:Tokyo_Metropolitan/Proj...) |
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | + | *4-5 | |
| - | + | *9-7 | |
| + | *vector | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ] | ||
| - | |||
===2:Electrophoresis=== | ===2:Electrophoresis=== | ||
Revision as of 11:24, 16 October 2010
Contents |
2010/10/15
1：DNA extraction
＜Member＞
Hitomi
＜Sample＞
- 4-5
- 9-7
- vector
＜Protocol＞
SeeProtocol 4
2:Electrophoresis
<Member>
Hitomi
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
3：DNA extraction from gels
＜Member＞
Mariko
＜Sample＞
- 1+2(8/31)
- 4+5(8/31)
(After electrophoresis)
＜Protocol＞
SeeProtocol 4
4:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 1-2
- 4-5
- 7-8-10
<Protocol>
See Protocol 2
5:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
6:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 1-2
- 4-5
- 7-8-10
<Protocol>
See Protocol 2
7：DNA Digestion
＜Member＞
＜Sample, Materials＞
・PCR productions
- 1L(8/25) 8μl
- 2L(8/26) 8μl
- 4H(8/26) 16μl
- 5H(8/26) 16μl
・Digest enzyme
- AvrⅡ
- NheⅠ
- SpeⅠ
＜Protocol＞
See Protocol 9
8：DNA Ligation
＜Member＞
＜Sample＞
- 1L(8/25)
- 2L(8/26)
- 4H(8/26)
- 5H(8/26)
(after digestion)
＜Protocol＞
See Protocol 3
9:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 1-2
- 4-5
- 7-8-10
<Protocol>
See Protocol 2
10:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 1-2
- 4-5
- 7-8-10
<Protocol>
See Protocol 2
11:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
12:Transformation
<Member>
nito
<Sample>
- 7-8-10
- JM109
<Protocol>
See Protocol 7
13:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
14:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products (9/16)
<Protocol>
See Protocol 8
<Result>
