Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12

From 2010.igem.org

(Difference between revisions)
(→5：DNA Ligation)
(→3：DNA Digestion)
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
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==3：DNA Digestion==
+
===3：DNA Digestion===
＜Member＞<br />
＜Member＞<br />
-
 
+
nito
＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
・PCR productions
・PCR productions
-
*1L(8/25)  8μl
+
*9H
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*2L(8/26)  8μl
+
*1-2
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*4H(8/26)  16μl
+
*10-2
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*5H(8/26)  16μl
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・Digest enzyme
・Digest enzyme

Revision as of 10:25, 16 October 2010

Contents

2010/10/12

1：DNA extraction

＜Member＞
Mariko

＜Sample＞

  • 1-2
  • 10-2
  • 2

＜Protocol＞
See Protocol 4

2：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR product

  • 1-2

・Digest enzyme

  • Avr2

＜Protocol＞
See Prptpcpl 9

3:PCR

<Member>
nito

<Sample>

  • 9
  • 1-2-3
  • 4-5

<Protocol>
See Protocol 2

4:Plasmid extraction from E-coli

<Member>
taka

<Sample>

  • 1-2
  • 1-3
  • 1-5
  • 1-6

<Protocol>
See Protocol 6

5：DNA Ligation

＜Member＞
nito

＜Sample＞

  • 1-2
  • 10-2


＜Protocol＞
See Protocol 10

3：DNA Digestion

＜Member＞
nito


＜Sample, Materials＞
・PCR productions

  • 9H
  • 1-2
  • 10-2

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • SpeⅠ


＜Protocol＞
See Protocol 9

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