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Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/12
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
| - | === | + | ===4:Plasmid extraction from E-coli=== |
<Member><br /> | <Member><br /> | ||
| - | + | taka | |
<Sample><br /> | <Sample><br /> | ||
| - | * | + | *1-2 |
| - | * | + | *1-3 |
| + | *1-5 | ||
| + | *1-6 | ||
<Protocol><br /> | <Protocol><br /> | ||
Revision as of 10:22, 16 October 2010
Contents |
2010/10/12
1：DNA extraction
＜Member＞
Mariko
＜Sample＞
- 1-2
- 10-2
- 2
＜Protocol＞
See Protocol 4
2：DNA Digestion
＜Member＞
Mariko
＜Sample, Materials＞
・PCR product
- 1-2
・Digest enzyme
- Avr2
＜Protocol＞
See Prptpcpl 9
3:PCR
<Member>
nito
<Sample>
- 9
- 1-2-3
- 4-5
<Protocol>
See Protocol 2
4:Plasmid extraction from E-coli
<Member>
taka
<Sample>
- 1-2
- 1-3
- 1-5
- 1-6
<Protocol>
See Protocol 6
5：DNA Ligation
＜Member＞
mio,mizuki
＜Sample＞
- 1-2
- 4-5
- 6A-7
- 7-8-10
(after digestion)
＜Protocol＞
See Protocol 10
3：DNA Digestion
＜Member＞
＜Sample, Materials＞
・PCR productions
- 1L(8/25) 8μl
- 2L(8/26) 8μl
- 4H(8/26) 16μl
- 5H(8/26) 16μl
・Digest enzyme
- AvrⅡ
- NheⅠ
- SpeⅠ
＜Protocol＞
See Protocol 9
