Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test28settembre
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Than we performed a 21 hours' experiment with measurements of absorbance and green fluorescence every five minutes with TECAN Infinite F200. I63 and I64 circuits were also induced HSL 100nM directly in multiplate well. Each value shown is the mean of three measurements; cultures were shaked for 15 seconds every five minutes. | Than we performed a 21 hours' experiment with measurements of absorbance and green fluorescence every five minutes with TECAN Infinite F200. I63 and I64 circuits were also induced HSL 100nM directly in multiplate well. Each value shown is the mean of three measurements; cultures were shaked for 15 seconds every five minutes. | ||
- | ==RESULTS | + | ==RESULTS== |
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+ | [[Image:UNIPV_Pavia_ASB28settembre.png| 500px | center ]] | ||
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+ | [[Image:UNIPV_Pavia_GFP28settembre.png| 500px | center ]] | ||
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+ | All induced and uninduced cell cultures showed a similar growth curve. After an initial interval in witch GFP production rate was significantly higher in positive control <partinfo>BBa_K173002</partinfo> than in the other samples, GFP synthesis rate of induced I63 and I64, I65 became very similar to this one. It was still lower than positive control, but was constant during all the experiment. This showed the right folding of the green fluorescent protein assembled downstream of the genetic circuit and the working of the HSL inducible system. As expected uninduced I63 and I64 didn't produce any GFP, behaving as negative controls. | ||
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Revision as of 22:41, 14 October 2010
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