Restriction Enzyme Digestion

Materials

Plasmid DNA: 10.5 µL/5 µL

Restriction Enzymes (depend on the requirements of the parts)

  EcoRI: 1 µL

  PstI: 1 µL

  SpeI: 1 µL

  XbaI: 1 µL

  10× NE buffer: 1.5 µL/1 µL (lid)

  BSA: 1 µL (lid)

Method

1. Add all the contents listed above to labelled eppendorfs.

2. Spin for 30 sec.

3. Incubation at 37°C for 1hr.

4. Leave on ice before the gel electrophoresis