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Team:Harvard/vectors
From 2010.igem.org
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<p>Our goal is the creation of an "iGarden" with the BioBrick standard. To approach this project, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli to agrobacterium, and finally into the plant arabidopsis.</p> | <p>Our goal is the creation of an "iGarden" with the BioBrick standard. To approach this project, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli to agrobacterium, and finally into the plant arabidopsis.</p> | ||
| - | <p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into | + | <p>The process began with the creation of desired constructs in e. coli - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms. We next built a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.</p> |
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<img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"> | <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"> | ||
Revision as of 17:23, 14 October 2010
abstract lab notebook agrobacteria and plant engineering meet the vectors plant transformation parts & primers references
abstract
Our goal is the creation of an "iGarden" with the BioBrick standard. To approach this project, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli to agrobacterium, and finally into the plant arabidopsis.
The process began with the creation of desired constructs in e. coli - such as flavor elements, allergen knockdown, pigment accumulation, or fence mechanisms. We next built a set of plant vectors compatible with the BioBrick standard and moved our parts from e. coli into these vectors, which allowed us to electroporate our parts into agrobacteria. The final step was to transform arabidopsis flowers with our engineered agrobacteria and select for plants containing our designed constructs.
