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From 2010.igem.org

Revision as of 11:45, 14 October 2010 (view source) AndreasConstantinou (Talk | contribs) ← Older edit		Revision as of 12:00, 14 October 2010 (view source) AndreasConstantinou (Talk | contribs) (→Andreas) Newer edit →
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	{{Stockholm/Top2}}		{{Stockholm/Top2}}
	==Andreas==		==Andreas==
		+	[[image:Birthdaycake.jpg|Birthday cake]]
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		+	'''Short day in lab - birthday celebrations!'''
	===Growth curve assay===		===Growth curve assay===

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Revision as of 12:00, 14 October 2010

Contents 1 Andreas 1.1 Growth curve assay 1.2 Removal of insertion in BioBrick suffixes 1.2.1 Plasmid prep

Andreas

Short day in lab - birthday celebrations!

Growth curve assay

Inoculated fresh LB with 0.3 mM IPTG with ON cultures to a startup OD of 0.2 and a total volume of 50 ml in 150 ml side-arm E-flasks.

Was advised to use a smaller cultures for the flask size. Experiment canceled after about an hour; will be repeated next week.

Removal of insertion in BioBrick suffixes

Plasmid prep

Spun down pSB1C3.SOD ON culture at 13,000 x g, 10 min. Supernatant discarded and cell pellet stored in -20 °C for later plasmid prep.