Team:LMU-Munich/ProSearch

From 2010.igem.org

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{{:Team:LMU-Munich/Templates/Page Header}}
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[[Image:prosearchZelle.jpg|300px|Logo|right]]
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=ProSearch System=
 
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This system is to search for new enhancer-promoter combinations that efficiently trigger gene expression in response to different stimuli. This part of the project is carried out through a bioinformatic modeling process. The end-product is a databank of related genes, their promoters and transcription factors at the input of a wished environmental condition. This system is useful for the construction of new inducible systems trying to utilize apoptosis or other gene expression in the human cell-line 293T.
 
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#!/bin/sh
 
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echo Content-type: text/html
 
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echo ""
 
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### $QUERY_STRING=namex=valuex&namey=valuey&namez=valuez
 
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QS=`echo $QUERY_STRING | sed -e s/\\\`//g -e s/\"//g`
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==ProSearch System==
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str1=`echo $QS|awk -F\& '{printf $1}'`
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This system is to search for new enhancer-promoter combinations that efficiently trigger gene expression in response to different stimuli. This part of the project is carried out through a bioinformatic modeling process. The end-product is a databank of related genes, their promoters and transcription factors at the input of a wished environmental condition. This system is useful for the construction of new inducible systems trying to utilize apoptosis or other gene expression in the human cell-line 293T.
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str2=`echo $QS|awk -F\& '{printf $2}'`
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arg1=`echo $str1|awk -F= '{printf $2}'`
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arg2=`echo $str2|awk -F= '{printf $2}'`
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/bin/cat << EOM
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<HTML>
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<HEAD><TITLE>Transcription Factors of genes differentially expressed in condition $arg1 </TITLE>
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</HEAD>
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<BODY bgcolor="#cccccc" text="#000000">
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<HR SIZE=5>
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<H1>TFs of genes differentially expressed in condition $arg1</H1>
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<HR SIZE=5>
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<P>
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<SMALL>
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<PRE>
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EOM
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java GetTFsPromoters "$arg1" "$arg2"
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cat << EOM
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==Retrieve Genes, their Promoters and Transcription Factors for a specific condition==
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</PRE>
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<html>
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</SMALL>
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<body>
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<P>
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<form action="http://ka0.org/igem/getTF.cgi" method="get">  
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</BODY>
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<p>Condition<br>
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</HTML>
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<SELECT NAME="condition">
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EOM
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<OPTION VALUE="beta-catenin">bCatenin treatment</OPTION>  
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<OPTION VALUE="conduritolB">conduritol B treatment</OPTION>  
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<OPTION VALUE="gHerpes">gHerpes infection</OPTION>  
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<OPTION VALUE="hiv">HIV infection</OPTION>  
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<OPTION VALUE="hypoxia">hypoxia</OPTION>
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<OPTION VALUE="htlv">HTLV infection</OPTION>
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</SELECT>
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<p>Specific Gene (leave empty for all):<br><input type="text" name="gene" size="50" maxlength="50"></p>
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<p><input type="submit" value="Send Query"></p>
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</form>
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</body>
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</html>
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Latest revision as of 14:43, 25 October 2010


Logo



ProSearch System

This system is to search for new enhancer-promoter combinations that efficiently trigger gene expression in response to different stimuli. This part of the project is carried out through a bioinformatic modeling process. The end-product is a databank of related genes, their promoters and transcription factors at the input of a wished environmental condition. This system is useful for the construction of new inducible systems trying to utilize apoptosis or other gene expression in the human cell-line 293T.

Retrieve Genes, their Promoters and Transcription Factors for a specific condition

Condition

Specific Gene (leave empty for all):
 

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